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Use of a proposed antimicrobial susceptibility testing method for Haemophilus parasuis

Dayao, Denise Ann E. and Kienzle, Marco and Gibson, Justine S. and Blackall, Patrick J. and Turni, Conny (2014) Use of a proposed antimicrobial susceptibility testing method for Haemophilus parasuis. Veterinary Microbiology, 172 (3-4). p. 586. ISSN 03781135

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Article Link(s): http://dx.doi.org/10.1016/j.vetmic.2014.06.010

Abstract

The aim of this study was to examine the antimicrobial susceptibility of 97 Haemophilus parasuis cultured from Australian pigs. As there is no existing standard antimicrobial susceptibility technique available for H. parasuis, methods utilising the supplemented media, BA/SN for disc diffusion and test medium broth (TMB) for a microdilution technique, were initially evaluated with the reference strains recommended by the Clinical and Laboratory Standards Institute. The results of the media evaluation suggested that BA/SN and TMB can be used as suitable media for susceptibility testing of H. parasuis. The proposed microdilution technique was then used with 97 H. parasuis isolates and nine antimicrobial agents. The study found that Australian isolates showed elevated minimum inhibitory concentrations (MICs) for ampicillin (1%), penicillin (2%), erythromycin (7%), tulathromycin (9%), tilmicosin (22%), tetracycline (31%) and trimethoprim-sulfamethoxazole (40%). This study has described potential antimicrobial susceptibility methods for H. parasuis and has detected a low percentage of Australian H. parasuis isolates with elevated antimicrobial MICs.

Item Type:Article
Business groups:Animal Science
Subjects:Animal culture > Swine
Veterinary medicine > Veterinary microbiology
Deposited On:25 Aug 2014 04:30
Last Modified:25 Aug 2014 04:30

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