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Applications of a sugar-based surveillance system to track arboviruses in wild mosquito populations

Van Den Hurk, A. F. and Hall-Mendelin, S. and Townsend, M. and Kurucz, N. and Edwards, J. and Ehlers, G. and Rodwell, C. and Moore, F. A. and McMahon, J. L. and Northill, J. A. and Simmons, R. J. and Cortis, G. and Melville, L. and Whelan, P. I. and Ritchie, S. A. (2014) Applications of a sugar-based surveillance system to track arboviruses in wild mosquito populations. Vector-Borne and Zoonotic Diseases, 14 (1). pp. 66-73. ISSN 15303667 (ISSN)

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Article Link(s): http://dx.doi.org/10.1089/vbz.2013.1373.

Abstract

Effective arbovirus surveillance is essential to ensure the implementation of control strategies, such as mosquito suppression, vaccination, or dissemination of public warnings. Traditional strategies employed for arbovirus surveillance, such as detection of virus or virus-specific antibodies in sentinel animals, or detection of virus in hematophagous arthropods, have limitations as an early-warning system. A system was recently developed that involves collecting mosquitoes in CO2-baited traps, where the insects expectorate virus on sugar-baited nucleic acid preservation cards. The cards are then submitted for virus detection using molecular assays. We report the application of this system for detecting flaviviruses and alphaviruses in wild mosquito populations in northern Australia. This study was the first to employ nonpowered passive box traps (PBTs) that were designed to house cards baited with honey as the sugar source. Overall, 20/144 (13.9%) of PBTs from different weeks contained at least one virus-positive card. West Nile virus Kunjin subtype (WNVKUN), Ross River virus (RRV), and Barmah Forest virus (BFV) were detected, being identified in 13/20, 5/20, and 2/20 of positive PBTs, respectively. Importantly, sentinel chickens deployed to detect flavivirus activity did not seroconvert at two Northern Territory sites where four PBTs yielded WNVKUN. Sufficient WNVKUN and RRV RNA was expectorated onto some of the honey-soaked cards to provide a template for gene sequencing, enhancing the utility of the sugar-bait surveillance system for investigating the ecology, emergence, and movement of arboviruses. © 2014, Mary Ann Liebert, Inc.

Item Type:Article
Business groups:Biosecurity Queensland
Keywords:Arbovirus Control-Australia Mosquito Surveillance sugar virus RNA article Barmah Forest virus nonhuman priority journal Ross River alpha virus West Nile flavivirus West Nile virus Kunjin subtype
Subjects:Veterinary medicine > Veterinary virology
Science > Entomology
Deposited On:05 Aug 2014 04:29
Last Modified:05 Aug 2014 04:29

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