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Seminal plasma proteome of eletroejaculated Bos indicus bulls

Rego, J. P. A. and Crisp, J. M. and Moura, A. A. and Nouwens, A. S. and Li, Y. and Venus, B. and Corbet, N. J. and Corbet, D. H. and Burns, B. M. and Boe-Hansen, G. B. and McGowan, M. R. (2014) Seminal plasma proteome of eletroejaculated Bos indicus bulls. Animal Reproduction Science . ISSN 0378-4320

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Article Link(s): http://dx.doi.org/10.1016/j.anireprosci.2014.04.01...

Publisher URL: http://www.sciencedirect.com/science/article/pii/S0378432014001353

Abstract

The present study describes the seminal plasma proteome of Bos indicus bulls. Fifty-six, 24-month old Australian Brahman sires were evaluated and subjected to electroejaculation. Seminal plasma proteins were separated by 2-D SDS-PAGE and identified by mass spectrometry. The percentage of progressively motile and morphologically normal sperm of the bulls were 70.4±2.3 and 64±3.2%, respectively. A total of 108 spots were identified in the 2-D maps, corresponding to 46 proteins. Binder of sperm proteins accounted for 55.8% of all spots detected in the maps and spermadhesins comprised the second most abundant constituents. Other proteins of the Bos indicus seminal plasma include clusterin, albumin, transferrin, metalloproteinase inhibitor 2, osteopontin, epididymal secretory protein E1, apolipoprotein A-1, heat shock 70kDa protein, glutathione peroxidase 3, cathelicidins, alpha-enolase, tripeptidyl-peptidase 1, zinc-alpha-2-glycoprotein, plasma serine protease inhibitor, beta 2-microglobulin, proteasome subunit beta type-4, actin, cathepsins, nucleobinding-1, protein S100-A9, hemoglobin subunit alpha, cadherin-1, angiogenin-1, fibrinogen alpha and beta chain, ephirin-A1, protein DJ-1, serpin A3-7, alpha-2-macroglobulin, annexin A1, complement factor B, polymeric immunoglobulin receptor, seminal ribonuclease, ribonuclease-4, prostaglandin-H2 D-isomarase, platelet-activating factor acetylhydrolase, and phosphoglycerate kinase In conclusion, this work uniquely portrays the Bos indicus seminal fluid proteome, based on samples from a large set of animals representing the Brahman cattle of the tropical Northern Australia. Based on putative biochemical attributes, seminal proteins act during sperm maturation, protection, capacitation and fertilization.

Item Type:Article
Business groups:Animal Science
Keywords:Proteome Electrophoresis Sperm Bos indicus Seminal plasma
Subjects:Science > Biology > Reproduction
Animal culture > Cattle
Deposited On:05 Aug 2014 06:11
Last Modified:05 Aug 2014 06:11

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