Login | Create Account (DAF staff only)

Fungal community structure in disease suppressive soils assessed by 28S LSU gene sequencing

Penton, C. R. and Gupta, V. V. S. R. and Tiedje, J. M. and Neate, S. M. and Ophel-Keller, K. and Gillings, M. and Harvey, P. and Pham, A. and Roget, D. K. (2014) Fungal community structure in disease suppressive soils assessed by 28S LSU gene sequencing. PLoS ONE, 9 (4). ISSN 19326203

Full text not currently attached. Access may be available via the Publisher's website or OpenAccess link.

Article Link(s): http://dx.doi.org/10.1371/journal.pone.0093893

Abstract

Natural biological suppression of soil-borne diseases is a function of the activity and composition of soil microbial communities. Soil microbe and phytopathogen interactions can occur prior to crop sowing and/or in the rhizosphere, subsequently influencing both plant growth and productivity. Research on suppressive microbial communities has concentrated on bacteria although fungi can also influence soil-borne disease. Fungi were analyzed in co-located soils 'suppressive' or 'non-suppressive' for disease caused by Rhizoctonia solani AG 8 at two sites in South Australia using 454 pyrosequencing targeting the fungal 28S LSU rRNA gene. DNA was extracted from a minimum of 125 g of soil per replicate to reduce the micro-scale community variability, and from soil samples taken at sowing and from the rhizosphere at 7 weeks to cover the peak Rhizoctonia infection period. A total of ∼994,000 reads were classified into 917 genera covering 54% of the RDP Fungal Classifier database, a high diversity for an alkaline, low organic matter soil. Statistical analyses and community ordinations revealed significant differences in fungal community composition between suppressive and non-suppressive soil and between soil type/location. The majority of differences associated with suppressive soils were attributed to less than 40 genera including a number of endophytic species with plant pathogen suppression potentials and mycoparasites such as Xylaria spp. Non-suppressive soils were dominated by Alternaria , Gibberella and Penicillum. Pyrosequencing generated a detailed description of fungal community structure and identified candidate taxa that may influence pathogen-plant interactions in stable disease suppression. © 2014 Penton et al.

Item Type:Article
Business groups:Crop and Food Science
Subjects:Plant pests and diseases > Pest control and treatment of diseases. Plant protection > Soil disinfection
Plant pests and diseases > Plant pathology
Agriculture > Agriculture (General) > Soils. Soil science
Deposited On:19 Aug 2014 01:58
Last Modified:19 Aug 2014 01:58

Repository Staff Only: item control page