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Freeze-drying of ovalbumin loaded mesoporous silica nanoparticle vaccine formulation increases antigen stability under ambient conditions

Mody, K. T., Mahony, D., Cavallaro, A. S., Stahr, F., Qiao, S. Z., Mahony, T. J. and Mitter, N. (2014) Freeze-drying of ovalbumin loaded mesoporous silica nanoparticle vaccine formulation increases antigen stability under ambient conditions. International Journal of Pharmaceutics . ISSN 0378-5173

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Article Link(s): http://dx.doi.org/10.1016/j.ijpharm.2014.01.037

Abstract

Amino functionalised mesoporous silica nanoparticles (AM-41) have been identified as a promising vaccine delivery material. The capacity of AM-41 to stabilise vaccine components at ambient temperature (23–27 °C) was determined by adsorbing the model antigen ovalbumin (OVA) to AM-41 particles (OVA-41). The OVA-41 was successfully freeze-dried using the excipients 5% trehalose and 1% PEG8000. Both the immunological activity of OVA and the nanoparticle structure were maintained following two months storage at ambient temperature. The results of immunisation studies in mice with reconstituted OVA-41 demonstrated the induction of humoral and cell-meditated immune responses. The capacity of AM-41 particles to facilitate ambient storage of vaccine components without loss of immunological potency will underpin the further development of this promising vaccine delivery platform.

Item Type:Article
Business groups:Animal Science
Keywords:Mesoporous silica nanoparticles; Protein; Freeze-drying; Excipients; Stability; Vaccine delivery
Subjects:Veterinary medicine > Veterinary pharmacology
Deposited On:19 Aug 2014 03:05
Last Modified:19 Aug 2014 03:05

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