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Freeze-drying of ovalbumin loaded mesoporous silica nanoparticle vaccine formulation increases antigen stability under ambient conditions

Mody, Karishma T and Mahony, Donna and Cavallaro, Antonino S and Stahr, Frances and Qiao, Shi Zhang and Mahony, Timothy J and Mitter, Neena (2014) Freeze-drying of ovalbumin loaded mesoporous silica nanoparticle vaccine formulation increases antigen stability under ambient conditions. International Journal of Pharmaceutics . ISSN 0378-5173

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Article Link(s): http://dx.doi.org/10.1016/j.ijpharm.2014.01.037

Abstract

Amino functionalised mesoporous silica nanoparticles (AM-41) have been identified as a promising vaccine delivery material. The capacity of AM-41 to stabilise vaccine components at ambient temperature (23–27 °C) was determined by adsorbing the model antigen ovalbumin (OVA) to AM-41 particles (OVA-41). The OVA-41 was successfully freeze-dried using the excipients 5% trehalose and 1% PEG8000. Both the immunological activity of OVA and the nanoparticle structure were maintained following two months storage at ambient temperature. The results of immunisation studies in mice with reconstituted OVA-41 demonstrated the induction of humoral and cell-meditated immune responses. The capacity of AM-41 particles to facilitate ambient storage of vaccine components without loss of immunological potency will underpin the further development of this promising vaccine delivery platform.

Item Type:Article
Business groups:Animal Science
Keywords:Mesoporous silica nanoparticles; Protein; Freeze-drying; Excipients; Stability; Vaccine delivery
Subjects:Veterinary medicine > Veterinary pharmacology
Deposited On:19 Aug 2014 03:05
Last Modified:19 Aug 2014 03:05

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