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The rph2 Gene Is Responsible for High Level Resistance to Phosphine in Independent Field Strains of Rhyzopertha dominica

Mau, Y. S. and Collins, P. J. and Daglish, G. J. and Nayak, M. K. and Ebert, P. R. (2012) The rph2 Gene Is Responsible for High Level Resistance to Phosphine in Independent Field Strains of Rhyzopertha dominica. Plos One, 7 (3). ISSN 1932-6203

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Article Link(s): http://dx.doi.org/10.1371/journal.pone.0034027

Abstract

The lesser grain borer Rhyzopertha dominica (F.) is one of the most destructive insect pests of stored grain. This pest has been controlled successfully by fumigation with phosphine for the last several decades, though strong resistance to phosphine in many countries has raised concern about the long term usefulness of this control method. Previous genetic analysis of strongly resistant (SR) R. dominica from three widely geographically dispersed regions of Australia, Queensland (SRQLD), New South Wales (SRNSW) and South Australia (SRSA), revealed a resistance allele in the rph1 gene in all three strains. The present study confirms that the rph1 gene contributes to resistance in a fourth strongly resistant strain, SR2(QLD), also from Queensland. The previously described rph2 gene, which interacts synergistically with rph1 gene, confers strong resistance on SRQLD and SRNSW. We now provide strong circumstantial evidence that weak alleles of rph2, together with rph1, contribute to the strong resistance phenotypes of SRSA and SR2(QLD). To test the notion that rph1 and rph2 are solely responsible for the strong resistance phenotype of all resistant R. dominica, we created a strain derived by hybridising the four strongly resistant lines. Following repeated selection for survival at extreme rates of phosphine exposure, we found only slightly enhanced resistance. This suggests that a single sequence of genetic changes was responsible for the development of resistance in these insects.

Item Type:Article
Business groups:Crop and Food Science
Additional Information:Mau, Yosep S. Collins, Patrick J. Daglish, Gregory J. Nayak, Manoj K. Ebert, Paul R.
Subjects:Plant culture > Field crops > Grain. Cereals
Plant pests and diseases
Deposited On:18 Mar 2014 04:28
Last Modified:18 Mar 2014 04:28

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