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Origin of leaf rust adult plant resistance gene Rph20 in barley

Hickey, L. T. and Lawson, W. and Platz, G. J. and Dieters, M. and Franckowiak, J. (2012) Origin of leaf rust adult plant resistance gene Rph20 in barley. Genome, 55 (5). pp. 396-399. ISSN 0831-2796

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Article Link(s): http://dx.doi.org/10.1139/g2012-022

Abstract

Rph20 is the only reported, simply inherited gene conferring moderate to high levels of adult plant resistance (APR) to leaf rust (Puccinia hordei Otth) in barley (Hordeum vulgare L.). Key parental genotypes were examined to determine the origin of Rph20 in two-rowed barley. The Dutch cultivar 'Vada' (released in the 1950s) and parents, 'Hordeum laevigatum' and 'Gull' ('Gold'), along with the related cultivar 'Emir' (a derivative of 'Delta'), were assessed for APR to P. hordei in a disease screening nursery. The marker bPb-0837-PCR, co-located with Rph20 on the short arm of chromosome 5H (5HS), was used to screen genotypes for the resistance allele, Rph20.ai. Results from phenotypic assessment and DNA analysis confirmed that Rph20 originated from the landrace 'H. laevigatum' (i.e., Hordeum vulgare subsp. vulgare). Tracing back this gene through the pedigrees of two-rowed barley cultivars, indicated that Rph20 has contributed APR to P. hordei for more than 60 years. Although there have been no reports of an Rph20-virulent pathotype, the search for alternative sources of APR should continue to avoid widespread reliance upon a single resistance factor.

Item Type:Article
Business groups:Crop and Food Science
Additional Information:Hickey, Lee T. Lawson, Wendy Platz, Greg J. Dieters, Mark Franckowiak, Jerome
Subjects:Plant culture > Field crops > Barley
Plant pests and diseases > Individual or types of plants or trees > Barley
Science > Botany > Genetics
Deposited On:09 Apr 2014 04:55
Last Modified:19 Aug 2014 06:04

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