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Detection of a putative hemolysin operon, hhdBA, of Haemophilus parasuis from pigs with Glasser disease

Assavacheep, P. and Assavacheep, A. and Turni, C. (2012) Detection of a putative hemolysin operon, hhdBA, of Haemophilus parasuis from pigs with Glasser disease. Journal of Veterinary Diagnostic Investigation, 24 (2). pp. 339-343. ISSN 1040-6387

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Article Link(s): http://dx.doi.org/10.1177/1040638711435805

Abstract

The aim of the current study was to investigate whether polymerase chain reaction amplification of 16S ribosomal (r)RNA and a putative hemolysin gene operon, hhdBA, can be used to monitor live pigs for the presence of Haemophilus parasuis and predict the virulence of the strains present. Nasal cavity swabs were taken from 30 live, healthy, 1- to 8-week-old pigs on a weekly cycle from a commercial Thai nursery pig herd. A total of 27 of these pigs (90%) tested positive for H. parasuis as early as week 1 of age. None of the H. parasuis-positive samples from healthy pigs was positive for the hhdBA genes. At the same pig nursery, swab samples from nasal cavity, tonsil, trachea, and lung, and exudate samples from pleural/peritoneal cavity were taken from 30 dead pigs displaying typical pathological lesions consistent with Glasser disease. Twenty-two of 140 samples (15.7%) taken from 30 diseased pigs yielded a positive result for H. parasuis. Samples from the exudate (27%) yielded the most positive results, followed by lung, tracheal swab, tonsil, and nasal swab, respectively. Out of 22 positive samples, 12 samples (54.5%) harbored hhdA and/or hhdB genes. Detection rates of hhdA were higher than hhdB. None of the H. parasuis-positive samples taken from nasal cavity of diseased pigs tested positive for hhdBA genes. More work is required to determine if the detection of hhdBA genes is useful for identifying the virulence potential of H. parasuis field isolates.

Item Type:Article
Business groups:Animal Science
Additional Information:ISI Document Delivery No.: 983JB Times Cited: 1 Cited Reference Count: 24 Assavacheep, Pornchalit Assavacheep, Anongnart Turni, Conny Chulalongkorn University Veterinary Science Research Fund [RG10/2553] The author(s) disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was funded by the Chulalongkorn University Veterinary Science Research Fund RG10/2553. Sage publications inc Thousand oaks
Keywords:Glasser disease Haemophilus parasuis hhdBA pigs pcr test in-vivo genes identification hybridization expression proteins strains sites iron
Subjects:Animal culture > Swine
Veterinary medicine > Communicable diseases of animals (General)
Veterinary medicine > Veterinary pathology
Deposited On:10 Apr 2014 05:12
Last Modified:10 Apr 2014 05:12

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