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Mango (Mangifera indica L.) peel extract fractions from different cultivars differentially affect lipid accumulation in 3T3-L1 adipocyte cells

Taing, M. W. and Pierson, J. T. and Shaw, P. N. and Dietzgen, R. G. and Roberts-Thomson, S. J. and Gidley, M. J. and Monteith, G. R. (2013) Mango (Mangifera indica L.) peel extract fractions from different cultivars differentially affect lipid accumulation in 3T3-L1 adipocyte cells. Food & Function, 4 (3). pp. 481-491. ISSN 2042-6496

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Article Link(s): htttp://dx.do.org/10.1039/c2fo30224a

Abstract

Plant phytochemicals are increasingly recognised as sources of bioactive molecules which may have potential benefit in many health conditions. In mangoes, peel extracts from different cultivars exhibit varying effects on adipogenesis in the 3T3-L1 adipocyte cell line. In this study, the effects of preparative HPLC fractions of methanol peel extracts from Irwin, Nam Doc Mai and Kensington Pride mangoes were evaluated. Fraction 1 contained the most hydrophilic components while subsequent fractions contained increasingly more hydrophobic components. High content imaging was used to assess mango peel fraction effects on lipid accumulation, nuclei count and nuclear area in differentiating 3T3-L1 cells. For all three mango cultivars, the more hydrophilic peel fractions 1-3 inhibited lipid accumulation with greater potency than the more hydrophobic peel fractions 4. For all three cultivars, the more lipophilic fraction 4 had concentrations that enhanced lipid accumulation greater than fractions 1-3 as assessed by lipid droplet integrated intensity. The potency of this fraction 4 varied significantly between cultivars. Using mass spectrometry, five long chain free fatty acids were detected in fraction 4; these were not present in any other peel extract fractions. Total levels varied between cultivars, with Irwin fraction 4 containing the highest levels of these free fatty acids. Lipophilic components appear to be responsible for the lipid accumulation promoting effects of some mango extracts and are the likely cause of the diverse effects of peel extracts from different mango cultivars on lipid accumulation.

Item Type:Article
Business groups:Horticulture and Forestry Science
Additional Information:ISI Document Delivery No.: 095SI Times Cited: 0 Cited Reference Count: 57 Taing, Meng-Wong Pierson, Jean-Thomas Shaw, Paul N. Dietzgen, Ralf G. Roberts-Thomson, Sarah J. Gidley, Michael J. Monteith, Gregory R. Australian Postgraduate Award; Australian Research Council [LP0883644] This work was supported in part by an Australian Postgraduate Award to MWT and an Australian Research Council Linkage grant (LP0883644). We thank Dr Ian Bally for the provision of mango fruit varieties from the Australian Mango GenBank. Royal soc chemistry Cambridge
Keywords:breast-cancer cells chain-fatty-acids ppar-gamma genetic diversity adipogenesis phytochemicals apoptosis fruit genistein obesity
Subjects:Plant culture > Food crops
Plant culture > Fruit and fruit culture
Plant culture
Deposited On:03 Sep 2013 04:52
Last Modified:03 Sep 2013 04:52

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