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Strong resistance to phosphine in the rusty grain beetle, Cryptolestes ferrugineus (Stephens) (Coleoptera: Laemophloeidae): its characterisation, a rapid assay for diagnosis and its distribution in Australia

Nayak, M. K. and Holloway, J. C. and Emery, R. N. and Pavic, H. and Bartlet, J. and Collins, P. J. (2012) Strong resistance to phosphine in the rusty grain beetle, Cryptolestes ferrugineus (Stephens) (Coleoptera: Laemophloeidae): its characterisation, a rapid assay for diagnosis and its distribution in Australia. Pest Management Science, 69 (1). pp. 48-53. ISSN 1526-498X

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Article Link(s): http://dx.doi.org/10.1002/ps.3360

Abstract

BACKGROUND: The recent development of very high resistance to phosphine in rusty grain beetle, Cryptolestes ferrugineus (Stephens), seriously threatens stored-grain biosecurity. The aim was to characterise this resistance, to develop a rapid bioassay for its diagnosis to support pest management and to document the distribution of resistance in Australia in 20072011. RESULTS: Bioassays of purified laboratory reference strains and field-collected samples revealed three phenotypes: susceptible, weakly resistant and strongly resistant. With resistance factors of > 1000 x , resistance to phosphine expressed by the strong resistance phenotype was higher than reported for any stored-product insect species. The new time-to-knockdown assay rapidly and accurately diagnosed each resistance phenotype within 6 h. Although less frequent in western Australia, weak resistance was detected throughout all grain production regions. Strong resistance occurred predominantly in central storages in eastern Australia. CONCLUSION: Resistance to phosphine in the rusty grain beetle is expressed through two identifiable phenotypes: weak and strong. Strong resistance requires urgent changes to current fumigation dosages. The development of a rapid assay for diagnosis of resistance enables the provision of same-day advice to expedite resistance management decisions. (c) 2012 Commonwealth of Australia. Published by John Wiley & Sons, Ltd.

Item Type:Article
Business groups:Horticulture and Forestry Science
Additional Information:Nayak, Manoj K. Holloway, Joanne C. Emery, Robert N. Pavic, Hervoika Bartlet, Justin Collins, Patrick J.
Subjects:Plant pests and diseases > Pest control and treatment of diseases. Plant protection
Plant culture > Field crops > Grain. Cereals
Deposited On:03 Sep 2013 06:01
Last Modified:03 Sep 2013 06:01

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