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A core metabolic enzyme mediates resistance to phosphine gas

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Schlipalius, D. I., Valmas, N., Tuck, A. G., Jagadeesan, R., Maddock, L., Kaur, R., Goldinger, A., Anderson, C., Kuang, J., Zuryn, S., Mau, Y.S., Cheng, Q., Collins, P. J., Nayak, M. K., Schirra, H. J., Hilliard, M. A. and Ebert, P. R. (2012) A core metabolic enzyme mediates resistance to phosphine gas. Science, 338 (6108). pp. 807-810. ISSN 1095-9203 (Electronic)0036-8075 (Linking)

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Article Link: http://dx.doi.org/10.1126/science.1224951

Publisher URL: http://www.sciencemag.org/content/338/6108/807.full


Phosphine is a small redox-active gas that is used to protect global grain reserves, which are threatened by the emergence of phosphine resistance in pest insects. We find that polymorphisms responsible for genetic resistance cluster around the redox-active catalytic disulfide or the dimerization interface of dihydrolipoamide dehydrogenase (DLD) in insects (Rhyzopertha dominica and Tribolium castaneum) and nematodes (Caenorhabditis elegans). DLD is a core metabolic enzyme representing a new class of resistance factor for a redox-active metabolic toxin. It participates in four key steps of core metabolism, and metabolite profiles indicate that phosphine exposure in mutant and wild-type animals affects these steps differently. Mutation of DLD in C. elegans increases arsenite sensitivity. This specific vulnerability may be exploited to control phosphine-resistant insects and safeguard food security.

Item Type:Article
Business groups:Crop and Food Science
Additional Information:Schlipalius, David I Valmas, Nicholas Tuck, Andrew G Jagadeesan, Rajeswaran Ma, Li Kaur, Ramandeep Goldinger, Anita Anderson, Cameron Kuang, Jujiao Zuryn, Steven Mau, Yosep S Cheng, Qiang Collins, Patrick J Nayak, Manoj K Schirra, Horst Joachim Hilliard, Massimo A Ebert, Paul R R01NS060129/NS/NINDS NIH HHS/United States Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't United States Science. 2012 Nov 9;338(6108):807-10. doi: 10.1126/science.1224951.
Keywords:Animals Arsenicals/pharmacology Arsenites/pharmacology Beetles/drug effects/ enzymology/genetics/metabolism Caenorhabditis elegans/drug effects/ enzymology/genetics/metabolism Caenorhabditis elegans Proteins/chemistry/genetics/metabolism Catalytic Domain Dihydrolipoamide Dehydrogenase/chemistry/ genetics/metabolism Insect Proteins/chemistry/genetics/metabolism Insecticide Resistance/ genetics Insecticides/pharmacology Metabolic Networks and Pathways Molecular Sequence Data Mutation Oxidation-Reduction Pesticides Phosphines/pharmacology Polymorphism, Genetic Protein Multimerization Tribolium/drug effects/ enzymology/genetics/metabolism
Subjects:Plant pests and diseases > Pest control and treatment of diseases. Plant protection
Plant culture > Field crops > Grain. Cereals
Live Archive:17 Sep 2013 02:04
Last Modified:12 Jan 2024 04:33

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