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A multisite managed environment facility for targeted trait and germplasm phenotyping

Rebetzke, G. J. and Chenu, K. and Biddulph, B. and Moeller, C. and Deery, D. M. and Rattey, A. R. and Bennett, D. and Barrett-Lennard, E. G. and Mayer, J. E. (2013) A multisite managed environment facility for targeted trait and germplasm phenotyping. Functional Plant Biology, 40 (1). pp. 1-13. ISSN 1445-4408

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Article Link(s): http://dx.doi.org/10.1071/FP12180

Publisher URL: http://www.publish.csiro.au/paper/FP12180.htm

Abstract

Field evaluation of germplasm for performance under water and heat stress is challenging. Field environments are variable and unpredictable, and genotype x environment interactions are difficult to interpret if environments are not well characterised. Numerous traits, genes and quantitative trait loci have been proposed for improving performance but few have been used in variety development. This reflects the limited capacity of commercial breeding companies to screen for these traits and the absence of validation in field environments relevant to breeding companies, and because little is known about the economic benefit of selecting one particular trait over another. The value of the proposed traits or genes is commonly not demonstrated in genetic backgrounds of value to breeding companies. To overcome this disconnection between physiological trait breeding and uptake by breeding companies, three field sites representing the main environment types encountered across the Australian wheatbelt were selected to form a set of managed environment facilities (MEFs). Each MEF manages soil moisture stress through irrigation, and the effects of heat stress through variable sowing dates. Field trials are monitored continuously for weather variables and changes in soil water and canopy temperature in selected probe genotypes, which aids in decisions guiding irrigation scheduling and sampling times. Protocols have been standardised for an essential core set of measurements so that phenotyping yield and other traits are consistent across sites and seasons. MEFs enable assessment of a large number of traits across multiple genetic backgrounds in relevant environments, determine relative trait value, and facilitate delivery of promising germplasm and high value traits into commercial breeding programs.

Item Type:Article
Business groups:Crop and Food Science
Additional Information:Rebetzke, Greg J. Chenu, Karine Biddulph, Ben Moeller, Carina Deery, Dave M. Rattey, Allan R. Bennett, Dion Barrett-Lennard, Ed G. Mayer, Jorge E. Grains Research and Development Corporation The authors thank the MEF coordinators and technical staff who have contributed to the on-site development of the three facilities. These include Alan Harrod, Rick Graham, Graeme Rapp and Andy Hundt. Thanks also to Drs Rana Munns, John Kirkegaard and John Passioura for comments on the manuscript; to Dr Chris Moore, wheat breeder with InterGrain Pty Ltd for discussions; and to the Australian commercial wheat breeders who provided support in the MEFs for the assessment of germplasm under water limitation. We also thank the Grains Research and Development Corporation for their strong support in the development and funding of activities contained in the MEF. Csiro publishing Collingwood
Keywords:commercial breeding field experiments heat stress water stress wheat carbon-isotope discrimination water-use efficiency grain-yield genetic-improvement drought tolerance wheat cultivars bread wheat high-temperature abiotic stress winter-wheat
Subjects:Science > Botany > Genetics
Plant culture > Field crops > Wheat
Agriculture > Agriculture (General) > Methods and systems of culture. Cropping systems
Deposited On:17 Sep 2013 02:47
Last Modified:08 Aug 2017 14:48

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