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Comparative analysis of Tritrichomonas foetus (Riedmuller, 1928) cat genotype, T. foetus (Riedmuller, 1928) cattle genotype and Tritrichomonas suis (Davaine, 1875) at 10 DNA loci

Slapeta, J. and Muller, N. and Stack, C. M. and Walker, G. and Lew-Tabor, A. and Tachezy, J. and Frey, C. F. (2012) Comparative analysis of Tritrichomonas foetus (Riedmuller, 1928) cat genotype, T. foetus (Riedmuller, 1928) cattle genotype and Tritrichomonas suis (Davaine, 1875) at 10 DNA loci. International Journal for Parasitology, 42 (13-14). pp. 1143-1149. ISSN 0020-7519

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Article Link(s): http://dx.doi.org/10.1016/j.ijpara.2012.10.004

Abstract

The parasitic protists in the genus Tritrichomonas cause significant disease in domestic cattle and cats. To assess the genetic diversity of feline and bovine isolates of Tritrichomonas foetus (Riedmuller, 1928) Wenrich and Emmerson, 1933, we used 10 different genetic regions, namely the protein coding genes of cysteine proteases 1,2 and 4-9 (CP1, 2, 4-9) involved in the pathogenesis of the disease caused by the parasite. The cytosolic malate dehydrogenase 1 (MDH1) and internal transcribed spacer region 2 of the rDNA unit (ITS2) were included as additional markers. The gene sequences were compared with those of Tritrichomonas suis (Davaine. 1875) Morgan and Hawkins, 1948 and Tritrichomonas mobilensis Culberson et al., 1986. The study revealed 100% identity for all 10 genes among all feline isolates (=T. foetus cat genotype), 100% identity among all bovine isolates (=T. foetus cattle genotype) and a genetic distinctness of 1% between the cat and cattle genotypes of T. foetus. The cattle genotype of T. foetus was 100% identical to T. suis at nine loci (CP1, 2,4-8, ITS2, MDH1). At CP9, three out of four T. suis isolates were identical to the T. foetus cattle genotype, while the T. suis isolate SUI-H3B sequence contained a single unique nucleotide substitution. Tritrichomonas mobilensis was 0.4% and 0.7% distinct from the cat and cattle genotypes of T. foetus, respectively. The genetic differences resulted in amino acid changes in the CP genes, most pronouncedly in CP2, potentially providing a platform for elucidation of genotype-specific host-pathogen interactions of T. foetus. On the basis of this data we judge T. suis and T. foetus to be subjective synonyms. For the first time, on objective nomenclatural grounds, the authority of T. suis is given to Davaine, 1875, rather than the commonly cited Gruby and Delafond, 1843. To maintain prevailing usage of T. foetus, we are suppressing the senior synomym T. suis Davaine, 1875 according to Article 23.9, because it has never been used as a valid name after 1899 and T. foetus is widely discussed as the cause of bovine trichomonosis. Thus bovine, feline and porcine isolates should all be given the name T. foetus. This promotes the stability of T. foetus for the veterinary and economically significant venereal parasite causing bovine trichomonosis. (C) 2012 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.

Item Type:Article
Business groups:Animal Science
Additional Information:Slapeta, Jan Mueller, Norbert Stack, Colin M. Walker, Giselle Lew-Tabor, Ala Tachezy, Jan Frey, Caroline F. Dorothy Minchin Bequest (Faculty of Veterinary Science, University of Sydney, Australia); School of Science and Health Research Seed Grant (University of Western Sydney, Australia); Swiss Federal Veterinary Office [1.10.02, 1.12.03]; Swissgenetics; Swiss National Science Foundation [PBBEP3_141435]; Czech Ministry of Education [MSM0021620858] We sincerely thank Professor Carlos M Campero (National Institute of Agrarian Technology, Argentina) and Dr. Marcelo Fort (Animal Health Laboratory, Argentina) for providing the Argentinean T. foetus isolates; Dr. Klaus Henning (Friedrich-Loeffler-Institut, Germany) for providing the feline German T. foetus isolate; and International Code of Zoological Nomenclature (ICZN) Commissioners, Daphne G. Fautin and David J. Patterson, for advice on interpretation of the ICZN. We thank Bronwyn Venus for preparation of the YVL-W strain DNA and Biosecurity Queensland, Australia (Department of Agriculture, Fisheries & Forestry) for provision of the YVL-W T. foetus strain. This study was funded by the Dorothy Minchin Bequest (Faculty of Veterinary Science, University of Sydney, Australia) and in part by the School of Science and Health Research Seed Grant (University of Western Sydney, Australia). The Tritrichomonas work of CFF is supported by the Swiss Federal Veterinary Office (1.10.02 and 1.12.03) and by Swissgenetics. CFF is supported by the Swiss National Science Foundation (PBBEP3_141435). JT is supported by the Czech Ministry of Education (MSM0021620858). Elsevier sci ltd Oxford
Keywords:Tritrichomonas Cow Pig Cat Trichomoniasis Cysteine protease Genotyping Protozoa experimental-infection cysteine protease saimiri-boliviensis epithelial-cells sequence diarrhea trichomoniasis mobilensis delafond genes
Subjects:Veterinary medicine > Veterinary parasitology
Deposited On:19 Nov 2013 04:34
Last Modified:19 Nov 2013 04:34

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