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The meleagrid herpesvirus 1 genome is partially resistant to transposition

Hall, R. N. and Meers, J. and Mitter, N. and Fowler, E. V. and Mahony, T. J. (2013) The meleagrid herpesvirus 1 genome is partially resistant to transposition. Avian Diseases, 57 (2 SUPPL. 1). pp. 380-386. ISSN 00052086

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Article Link(s): http://dx.doi.org/10.1637/10339-082912-Reg.1

Abstract

The propagation of herpesvirus genomes as infectious bacterial artificial chromosomes (iBAC) has enabled the application of highly efficient strategies to investigate gene function across the genome. One of these strategies, transposition, has been used successfully on a number of herpesvirus iBACs to generate libraries of gene disruption mutants. Gene deletion studies aimed at determining the dispensable gene repertoire of the Meleagrid herpesvirus 1 (MeHV-1) genome to enhance the utility of this virus as a vaccine vector have been conducted in this report. A MeHV-1 iBAC was used in combination with the Tn5 and MuA transposition systems in an attempt to generate MeHV-1 gene interruption libraries. However, these studies demonstrated that Tn5 transposition events into the MeHV-1 genome occurred at unexpectedly low frequencies. Furthermore, characterization of genomic locations of the rare Tn5 transposon insertion events indicated a nonrandom distribution within the viral genome, with seven of the 24 insertions occurring within the gene encoding infected cell protein 4. Although insertion events with the MuA system occurred at higher frequency compared with the Tn5 system, fewer insertion events were generated than has previously been reported with this system. The characterization and distribution of these MeHV-1 iBAC transposed mutants is discussed at both the nucleotide and genomic level, and the properties of the MeHV-1 genome that could influence transposition frequency are discussed. © American Association of Avian Pathologists.

Item Type:Article
Business groups:Animal Science
Keywords:Bacterial artificial chromosome Herpesvirus of turkeys Mardivirus Meleagrid herpesvirus 1 MuA Mutagenesis Tn5 Transposition Turkey herpesvirus
Subjects:Veterinary medicine > Communicable diseases of animals (General)
Animal culture > Poultry > Turkeys
Deposited On:27 Nov 2013 06:39
Last Modified:27 Nov 2013 06:39

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