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Efficient organogenesis of an Australian passionfruit hybrid (Passiflora edulis x Passiflora edulis var. flavicarpa) suitable for gene delivery

Hall, R.M., Drew, R.A., Higgins, C.M. and Dietzgen, R.G. (2000) Efficient organogenesis of an Australian passionfruit hybrid (Passiflora edulis x Passiflora edulis var. flavicarpa) suitable for gene delivery. Australian Journal of Botany, 48 (5). pp. 673-680.

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Article Link(s): http://dx.doi.org/10.1071/BT99067

Publisher URL: http://www.publish.csiro.au

Abstract

An efficient regeneration protocol based on organogenesis from cotyledon explants and suitable for gene delivery has been developed for an Australian passionfruit hybrid. Multiple shoots were regenerated from 30-day-old cotyledon explants on Murashige and Skoog (MS) medium containing 6-benzylvaminopurine (BAP) and coconut water. Media pulsing experiments were conducted to investigate the effect on organogenesis of exposure time of the explants to MS containing 10 mu M BAP and 10% (v/v) coconut water, i.e. passionfruit regeneration medium (PRM). Continuous exposure of these explants to PRM maximised the number of shoots produced to 12.1 per explant. However, periods on hormone-free medium improved the appearance of the shoots and increased the number of explants with shoots from 75 to 84.6%. Further, shoots exposed for 7 days to half-strength MS supplemented with 10 mu M NAA (1-napthalene acetic acid) produced twice as many plantlets than those on half-strength MS alone. Transient GUS histochemical assays indicated delivery of the uidA gene via Agrobacterium tumefaciens.

Item Type:Article
Business groups:Crop and Food Science
Keywords:Passionfrunit hybrid; Passiflora; resistence; passionfruit cultivars; Incomplete; OVID-NEXT2; gene delivery.
Subjects:Science > Botany > Genetics
Plant culture > Fruit and fruit culture > Culture of individual fruits or types of fruit
Deposited On:05 Dec 2011 07:34
Last Modified:05 Dec 2018 23:10

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