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Evaluation of four serological tests for the diagnosis of caprine melioidosis

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Thomas, A. D., Spinks, G. A., D'Arcy, T. L., Norton, J. H. and Trueman, K. F. (1988) Evaluation of four serological tests for the diagnosis of caprine melioidosis. Australian Veterinary Journal, 65 (9). pp. 261-264.


Article Link: https://doi.org/10.1111/j.1751-0813.1988.tb16138.x


A complement fixation (CF) test, two indirect haemagglutination (IHA-A; IHA-L) tests which differed in antigen preparation and technique, and a microtitre agglutination (MA) test were compared in the serodiagnosis of melioidosis in goats. 118 experimental serum samples and 3143 field serum samples from goats in endemic and non-endemic areas of north Queensland were used. Culture of samples for Pseudomonas pseudomallei from 112 goats provided substantiating evidence of infection.
The IHA-A test was the most sensitive, and the CF test the most specific. It is recommended that the IHA-A be used as a screening test followed by the CF test for confirmation of active melioidosis. The IHA-A test is the better indicator of past infection. The four tests were compared using 577 serum samples from goats in areas where melioidosis is endemic; 42 were positive, 34 by IHA-A, 33 by MA and 30 by CFT.
Further tests are described using serum samples from 2480 goats grazing in endemic and non-endemic areas.

Item Type:Article
Corporate Creators:Biosecurity Queensland
Additional Information:Reproduced with permission from the © Australian Veterinary Journal. Access to published version may be available via Publisher’s website.
Keywords:Goat diseases; bacterial diseases; experimental infection; complement fixation tests; haemagglutination tests; agglutination tests; diagnosis; goats; Burkholderia pseudomallei; Australia; Pseudomonas pseudomallei; Capra; Bovidae; ruminant; Artiodactyla; Mammals; Vertebrates; Chordata; animals; Ungulates; Burkholderia; Burkholderiaceae; Gracilicutes; bacteria; prokaryotes; Australasia; Oceania; Developed Countries; Commonwealth of Nations; OECD Countries; Pseudomonadaceae.
Subjects:Veterinary medicine > Diseases of special classes of animals > Sheep and goats
Veterinary medicine > Veterinary bacteriology
Live Archive:26 Mar 2004
Last Modified:03 Sep 2021 16:43

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