Login | Request Account (DAF staff only)

Successful fertility experiments with cryopreserved spermatozoa of barramundi, Lates calcarifer (Bloch), using dimethylsulfoxide and glycerol as cryoprotectants

Share this record

Add to FacebookAdd to LinkedinAdd to XAdd to WechatAdd to Microsoft_teamsAdd to WhatsappAdd to Any

Export this record

View Altmetrics

Palmer, P.J., Blackshaw, A.W. and Garrett, R.N. (1993) Successful fertility experiments with cryopreserved spermatozoa of barramundi, Lates calcarifer (Bloch), using dimethylsulfoxide and glycerol as cryoprotectants. Reproduction, Fertility and Development, 5 (3). pp. 285-293.

[img]
Preview
PDF
455kB

Article Link: http://dx.doi.org/10.1071/RD9930285

Publisher URL: http://www.publish.csiro.au

Abstract

The fertility of cryopreserved Lates calcarifer sperm was studied to increase the availability of semen for routine fertilization of stripped eggs and to provide a tool for selective breeding. Semen diluted (1:4 v/v) and frozen (-196 degrees C) with 5% dimethylsulfoxide (DMSO) or 10% glycerol (final concentration) as cryoprotectants was used to inseminate freshly stripped ova. Frozen-thawed sperm were motile for about 4 min after being mixed with seawater. In the DMSO medium, post-thaw sperm activation was immediate after dilution with seawater, but in the glycerol medium maximum motility intensity was delayed for up to 1 min. When eggs and sperm were mixed before the addition of seawater, semen frozen with DMSO as cryoprotectant gave a mean hatch rate (84.1%) no different (P > 0.05) from that of unfrozen semen diluted with Ringer's solution (80.7%) or with DMSO (83.7%), but higher (P < 0.05) than that of semen frozen with glycerol (60.9%). Adding sperm to seawater 30 s before mixing with eggs did not improve the fertility of sperm cryopreserved with glycerol. Eggs inseminated with glycerol-cryoprotected sperm showed higher mortality during incubation than those inseminated with DMSO-cryoprotected sperm. Sperm held in liquid nitrogen for 90 days with DMSO as cryoprotectant yielded acceptable fertilization and hatching rates with semen-to-ova ratios of up to 1:100 (v/v) , and produced fish with no apparent abnormalities over a 29-day period after hatch. These results show that cryopreservation of L. calcarifer sperm is feasible and well suited to a variety of hatchery purposes.

Item Type:Article
Funders:Australian Research Council.
Corporate Creators:Queensland Department of Primary Industries, The University of Queensland, Agri-Science, DEEDI
Additional Information:© CSIRO. Reproduced with permission.
Keywords:Barramundi; sperm; cryopreservation; fertility
Subjects:Aquaculture and Fisheries > Aquaculture > Mariculture
Science > Biology
Live Archive:25 Aug 2011 06:59
Last Modified:03 Sep 2021 16:43

Repository Staff Only: item control page

Downloads

Downloads per month over past year

View more statistics