Outbreak of angular leaf spot, caused by Xanthomonas fragariae, in a Queensland strawberry germplasm collection.

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Young, A.J., Marney, T.S., Herrington, M. E., Hutton, D., Gomez, A., Villiers, A., Campbell, P. R. and Geering, A.D.W. (2011) Outbreak of angular leaf spot, caused by Xanthomonas fragariae, in a Queensland strawberry germplasm collection. Australasian Plant Pathology, 40 (3). pp. 286-292.

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Article Link: http://dx.doi.org/10.1007/s13313-011-0045-y

Abstract

Strawberry (Fragaria (x) ananassa) plants exhibiting leaf lesions consistent with angular leaf spot (ALS, caused by Xanthomonas fragariae Kennedy and King 1962) were identified in the Queensland strawberry germplasm at Bundeberg in May 2010. Water suspensions of bacterial ooze tested positive using a previously described primer set. However, the slow growth rate of X. fragariae and the presence of a fast-growing, non-pathogenic, undescribed Xanthomonas species presented problems that were overcome by dilution plating and DNA sequence analysis. Sequencing of the gyrB locus of putative colonies of X. fragariae indicated 100% sequence similarity to other X. fragariae isolates. A new set of diagnostic primers for X. fragariae based on the gyrB locus is presented.

Item Type:	Article
Business groups:	Crop and Food Science, Horticulture and Forestry Science
Additional Information:	© Australasian Plant Pathology Society Inc.
Keywords:	Diagnostic PCR; gyrB locus; cultural properties; strains; identification; arboricola; PRC; diversity; sequences; primers; disease; DNA; gene sequences; plants; leaf spot.
Subjects:	Plant culture > Fruit and fruit culture > Berries and small fruits Plant pests and diseases > Plant pathology Plant pests and diseases
Live Archive:	08 Jun 2011 02:04
Last Modified:	03 Nov 2022 04:02

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