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Characterization of highly informative cross-species microsatellite panels for the Australian dugong (Dugong dugon) and Florida manatee (Trichechus manatus latirostris) including five novel primers

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Hunter, M., Broderick, D., Ovenden, J.R., Tucker, K., Bonde, R.K., McGuire, P.M. and Lanyon, J. (2010) Characterization of highly informative cross-species microsatellite panels for the Australian dugong (Dugong dugon) and Florida manatee (Trichechus manatus latirostris) including five novel primers. Molecular Ecology Resources, 10 (2). pp. 368-377.

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Article Link: http://dx.doi.org/10.1111/j.1755-0998.2009.02761.x

Publisher URL: http://onlinelibrary.wiley.com/

Abstract

The Australian dugong (Dugong dugon) and Florida manatee (Trichechus manatus latirostris) are threatened species of aquatic mammals in the order Sirenia. Sirenian conservation and management actions would benefit from a more complete understanding of genetic diversity and population structure. Generally, species-specific microsatellite markers are employed in conservation genetic studies; however, robust markers can be difficult and costly to isolate. To increase the number of available markers, dugong and manatee microsatellite primers were evaluated for cross-species amplification. Furthermore, one manatee and four dugong novel primers are reported. After polymerase chain reaction optimization, 23 (92%) manatee primers successfully amplified dugong DNA, of which 11 (48%) were polymorphic. Of the 32 dugong primers tested, 27 (84%) yielded product in the manatee, of which 17 (63%) were polymorphic. Dugong and manatee primers were compared and the most informative markers were selected to create robust and informative marker-panels for each species. These cross-species microsatellite marker-panels can be employed to assess other sirenian populations and can provide beneficial information for the protection and management of these unique mammals.

Item Type:Article
Corporate Creators:DEEDI, Florida Integrated Science Center, University of Florida, University of South Florida, University of Queensland
Additional Information:Published 2009 This article is a US Government work and is in the public domain in the USA.
Keywords:Cross-species amplification; dugong; Dugong dugon; manatee; microsatellite primer; Trichechus manatus latirostris.
Subjects:Aquaculture and Fisheries > Fisheries
Science > Biology > Genetics
Science > Zoology > Chordates. Vertebrates
Live Archive:28 Apr 2011 07:29
Last Modified:03 Sep 2021 16:48

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