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Diagnosis of a naturally occurring dual infection of layer chickens with fowlpox virus and gallid herpesvirus 1 (infectious laryngotracheitis virus).

Diallo, I.S. and Taylor, J. and Gibson, J. and Hoad, J. and Jong, A.D. and Hewitson, G. and Corney, B.G. and Rodwell, B.J. (2010) Diagnosis of a naturally occurring dual infection of layer chickens with fowlpox virus and gallid herpesvirus 1 (infectious laryngotracheitis virus). Avian Pathology, 39 (1). pp. 25-30.

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Article Link(s): http://dx.doi.org/10.1080/03079450903447412

Publisher URL: http://www.taylorandfrancisgroup.com/

Abstract

An outbreak of acute respiratory disease in layers was diagnosed as being of dual nature due to fowlpox and infectious laryngotracheitis using a multidisciplinary approach including virus isolation, histopathology, electron microscopy and polymerase chain reaction (PCR). The diagnosis was based on virus isolation of gallid herpesvirus 1 (GaHV-1) in chicken kidney cells and fowlpox virus (FWPV) in 9-day-old chicken embryonated eggs inoculated via the chorioallantoic membrane. The histopathology of tracheas from dead birds revealed intra-cytoplasmic and intra-nuclear inclusions suggestive of poxvirus and herpesvirus involvement. The presence of FWPV was further confirmed by electron microscopy, PCR and histology. All FWPV isolates contained the long terminal repeats of reticuloendotheliosis virus as demonstrated by PCR. GaHV-1 isolates were detected by PCR and were shown to have a different restriction fragment length polymorphism pattern when compared with the chicken embryo origin SA2 vaccine strain; however, they shared the same pattern with the Intervet chicken embryo origin vaccine strain. This is a first report of dual infection of chickens with GaHV-1 and naturally occurring FWPV with reticuloendotheliosis virus insertions. Further characterization of the viruses was carried out and the results are reported here.

Item Type:Article
Corporate Creators:DEEDI, Biosecurity Queensland
Additional Information:© Taylor & Francis. © 2010 Houghton Trust Ltd.
Keywords:Animal diseases; characterization; diagnosis; eggs; embryos; epidemiology; fowl diseases; histology; histopathology; infections; infectious diseases; isolation; laryngotracheitis; lungs; mixed infections; outbreaks; pathology; polymerase chain reaction; polymorphism; poultry diseases; chickens; birds; respiratory diseases; restriction fragment length polymorphism; vaccines; viral diseases; communicable diseases; domesticated birds.
Subjects:Veterinary medicine > Diseases of special classes of animals > Avian
Veterinary medicine > Veterinary virology
Deposited On:23 Jun 2010 04:26
Last Modified:08 Sep 2010 05:01

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