"Extreme hybrids" from the Australian citrus rootstock breeding program

Abstract

Citrus is one of the most genetically diverse fruit trees used by humans, and yet few rootstock breeders have ventured beyond a narrow range of parents. For the last 25 years we have explored wider graft and sexual compatibility within the citrus subfamily (Aurantioideae) than any previous attempts, and have identified new genera and species for rootstock breeding. Whilst the vast majority of this work has met with failure, it has identified factors such as sexual incompatibility, late-acting lethality and poor field adaptation as the reasons why many citrus relatives are not currently amenable to rootstock breeding. A major breakthrough early in the program was the discovery that Oceania citrus species are extremely sensitive to Citrus tristeza virus (CTV) when used as rootstocks, and the realisation that resistance must be introgressed if we are to ever discover useful traits masked by this disease sensitivity. Bridging hybrids were required to transfer this virus resistance and a newly discovered species (Citrus wakonai) was employed to speed-up the process. After three generations of crossing, our "extreme hybrids" with Oceania parentage now show commercial performance equivalent to industry standards. By providing citrus growers with a range of high-performance rootstocks from extremely complex genetic backgrounds we can increase the biological diversity of orchards without compromising production. Our hope is that these "extreme hybrids" may help in the battle against Huanglongbing (HLB) disease. © 2024 Societa di Ortoflorofrutticoltura Italiana. All rights reserved.