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The assessment of TGGE for the detection of interspecific and intergeneric DNA-marker polymorphism within Solanaceae

Garland, S.H. (2005) The assessment of TGGE for the detection of interspecific and intergeneric DNA-marker polymorphism within Solanaceae. Australian Journal of Agricultural Research, 56 (3). pp. 291-299.

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Article Link(s): http://dx.doi.org/10.1071/AR04141

Publisher URL: http://www.publish.csiro.au/

Abstract

RFLP markers are currently the most appropriate marker system for the identification of uncharacterised polymorphism at the interspecific and intergeneric level. Given the benefits of a PCR-based marker system and the availability of sequence information for many Solanaceous cDNA clones, it is now possible to target conserved fragments, for primer development, that flank sequences possessing interspecific polymorphism. The potential outcome is the development of a suite of markers that amplify widely in Solanaceae. Temperature gradient gel electrophoresis (TGGE) is a relatively inexpensive gel-based system that is suitable for the detection of most single-base changes. TGGE can be used to screen for both known and unknown polymorphisms, and has been assessed here, for the development of PCR-based markers that are useful for the detection of interspecific variation within Solanaceae. Fifteen markers are presented where differences between Lycopersicon esculentum and L. pennellii have been detected by TGGE. The markers were assessed on a wider selection of plant species and found to be potentially useful for the identification of interspecific and intergeneric polymorphism in Solanaceous plants.

Item Type:Article
Additional Information:Reproduced with permission from © CSIRO Publishing. Access to published version may be available via Publisher’s website.
Keywords:Capsicum; pepper; potato; tomato; genetic marker; identification method; polymorphism; Lycopersicon esculentum; Lycopersicon pennellii; Magnoliophyta; Solanaceae; Solanum tuberosum.
Subjects:Plant culture > Food crops
Science > Biology > Genetics
Deposited On:06 Mar 2009 00:18
Last Modified:14 Mar 2011 07:01

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