Login | Request Account (DAF staff only)

Assessment of Bacillus licheniformis α-amylase as a candidate enzyme for genetic engineering of malting barley

Share this record

Add to FacebookAdd to LinkedinAdd to XAdd to WechatAdd to Microsoft_teamsAdd to WhatsappAdd to Any

Export this record

View Altmetrics

Vickers, J.E., Hamilton, S.E., De Jersey, J., Henry, R.J., Marschke, R.J. and Inkerman, P.A. (1996) Assessment of Bacillus licheniformis α-amylase as a candidate enzyme for genetic engineering of malting barley. Journal of the Institute of Brewing, 102 (2). pp. 75-78. ISSN 0046-9750

[img]
Preview
PDF
682kB

Article Link: https://doi.org/10.1002/j.2050-0416.1996.tb00896.x

Abstract

Bacillus licheniformis α-amylase, a thermostable starch-degrading enzyme, has been assessed as a candidate enzyme for the genetic transformation of malting barley. The temperature optimum, pH optimum and thermostability of B. licheniformis α-amylase were compared with those of barley α-amylase. The bacterial enzyme has a higher pH optimum (−9), a higher temperature optimum (−90°C) and much higher thermostability at elevated temperatures than the barley enzyme. The specific activity of the bacterial enzyme under conditions of pH and temperature relevant to the brewing process (pH 5.5, 65°C) is −1.5-fold higher than that of the barley enzyme. Measurements of α-amylase activity during a micro-mash showed that the bacterial enzyme is at least as stable as the barley enzyme under these conditions, and that a level of expression for the bacterial enzyme corresponding to −0.5% of total malt protein would approximately double the α-amylase activity in the mash. B. licheniformis α-amylase activity was rapidly eliminated by boiling following mashing as would occur during brewing. The combined results suggest that barley expressing the bacterial enzyme may be useful in the brewing process.

Item Type:Article
Subjects:Science > Botany > Genetics
Plant culture > Field crops > Barley
Live Archive:10 Apr 2024 03:09
Last Modified:10 Apr 2024 03:09

Repository Staff Only: item control page

Downloads

Downloads per month over past year

View more statistics