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Starch determination in horticultural plant material by an enzymic‐colorimetric procedure

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Rasmussen, T. S. and Henry, R. J. (1990) Starch determination in horticultural plant material by an enzymic‐colorimetric procedure. Journal of the Science of Food and Agriculture, 52 (2). pp. 159-170. ISSN 0022-5142

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Article Link: https://doi.org/10.1002/jsfa.2740520203


The starch content of horticultural plant material (including leaves and woody tissues such as roots, stems, twigs, trunks and bark) was determined by an enzymic procedure. A heat-stable α-amylase and amyloglucosidase were used to hydrolyse the starch in the finely ground plant material to glucose. The glucose produced was measured colorimetrically using a coupled glucose oxidase/peroxidase reaction. Conditions necessary for maximum glucose recovery were established. Plant material analysed included samples from macadamia (Macadamia integrifolia Maiden and Betche, trunk and bark), mango (Mangifera indica L; stems and roots), lychee (Litchi chinensis Sonn; leaves, stems, twigs and roots), pineapple (Ananas comosus (L) Merrill; leaves, stems and roots) and nectarine (Prunus persica (L) Batsch var nucipersica (Suckow) C K Schneid; twigs, shoots, branches, limbs, roots and trunk). Starch values ranged from less than 14% to greater than 30%. The method allows assessment of variations in the starch content of perennial plants and may be used in crop management.

Item Type:Article
Keywords:enzymic analysis; horticultural crops; Starch
Subjects:Agriculture > Agriculture (General) > Agricultural education > Research. Experimentation
Plant culture > Horticulture. Horticultural crops
Live Archive:16 Feb 2024 00:03
Last Modified:16 Feb 2024 00:03

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