A PCR assay for gender assignment in dugong (Dugong dugon) and West Indian manatee (Trichechus manatus)

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McHale, M., Broderick, D., Ovenden, J.R. and Lanyon, J.M. (2008) A PCR assay for gender assignment in dugong (Dugong dugon) and West Indian manatee (Trichechus manatus). Molecular Ecology Resources, 8 (3). pp. 669-670.

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Article Link: http://dx.doi.org/10.1111/j.1471-8286.2007.02041.x

Publisher URL: http://www3.interscience.wiley.com/cgi-bin/home

Abstract

Gender assignment for some aquatic mammals in the field is difficult. Molecular sexing from tissue biopsies is possible as males are heterogametic. Here we describe a multiplex PCR assay that amplifies the male specific SRY gene and differentiates ZFX and ZFY gametologues in two sirenian species, dugong (Dugong dugon) and West Indian manatee (Trichechus manatus). The assay was validated with animals of known gender and proved accurate and robust to experimental failure.

Item Type:	Article
Corporate Creators:	Fisheries
Additional Information:	© The State of Queensland Through its Department of Primary Industries and Fisheries.
Keywords:	Dugong; manatee; molecular sexing; SRY; ZFX; ZFY.
Subjects:	Aquaculture and Fisheries > Fisheries > Fishery research Science > Biology > Genetics Science > Zoology > Chordates. Vertebrates > Mammals
Live Archive:	02 Feb 2009 04:23
Last Modified:	03 Sep 2021 16:47

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