Login | Request Account (DAF staff only)

Fine-tuning for the tropics: application of eDNA technology for invasive fish detection in tropical freshwater ecosystems

View Altmetrics

Robson, H. L. A., Noble, T. H., Saunders, R. J., Robson, S. K. A., Burrows, D. W. and Jerry, D. R. (2016) Fine-tuning for the tropics: application of eDNA technology for invasive fish detection in tropical freshwater ecosystems. Molecular Ecology Resources, 16 (4). pp. 922-932. ISSN 1755-098X

Full text not currently attached. Access may be available via the Publisher's website or OpenAccess link.

Article Link: https://doi.org/10.1111/1755-0998.12505

Publisher URL: https://onlinelibrary.wiley.com/doi/abs/10.1111/1755-0998.12505


Abstract Invasive species pose a major threat to aquatic ecosystems. Their impact can be particularly severe in tropical regions, like those in northern Australia, where >20 invasive fish species are recorded. In temperate regions, environmental DNA (eDNA) technology is gaining momentum as a tool to detect aquatic pests, but the technology's effectiveness has not been fully explored in tropical systems with their unique climatic challenges (i.e. high turbidity, temperatures and ultraviolet light). In this study, we modified conventional eDNA protocols for use in tropical environments using the invasive fish, Mozambique tilapia (Oreochromis mossambicus) as a detection model. We evaluated the effects of high water temperatures and fish density on the detection of tilapia eDNA, using filters with larger pores to facilitate filtration. Large-pore filters (20 μm) were effective in filtering turbid waters and retaining sufficient eDNA, whilst achieving filtration times of 2–3 min per 2-L sample. High water temperatures, often experienced in the tropics (23, 29, 35 °C), did not affect eDNA degradation rates, although high temperatures (35 °C) did significantly increase fish eDNA shedding rates. We established a minimum detection limit for tilapia (1 fish/0.4 megalitres/after 4 days) and found that low water flow (3.17 L/s) into ponds with high fish density (>16 fish/0.4 megalitres) did not affect eDNA detection. These results demonstrate that eDNA technology can be effectively used in tropical ecosystems to detect invasive fish species.

Item Type:Article
Corporate Creators:Department of Agriculture and Fisheries, Queensland
Business groups:Animal Science
Subjects:Aquaculture and Fisheries
Aquaculture and Fisheries > Fisheries > By region or country > Australia > Great Barrier Reef
Aquaculture and Fisheries > Fisheries > Fishery research
Agriculture > By region or country > Australia > Queensland
Technology > Technology (General)
Live Archive:14 Feb 2024 01:28
Last Modified:14 Feb 2024 01:28

Repository Staff Only: item control page