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Isolation and characterization of microsatellite loci from mother-of-millions, Bryophyllum delagoense (Crassulaceae), and its hybrid with Bryophyllum daigremontianum, 'Houghton's hybrid'

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Hannan-Jones, M. A., Lowe, A.J., Scott, K. D., Graham, G.C., Playford, J.P. and Zalucki, M. P. (2005) Isolation and characterization of microsatellite loci from mother-of-millions, Bryophyllum delagoense (Crassulaceae), and its hybrid with Bryophyllum daigremontianum, 'Houghton's hybrid'. Molecular Ecology Notes, 5 (4). pp. 770-773. ISSN 1755-0998

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Article Link: https://doi.org/10.1111/j.1471-8286.2005.01058.x

Abstract

Nine microsatellite loci were developed, and are transferable, across the Madagascan succulents Bryophyllum daigremontianum, Bryophyllum delagoense (mother-of-millions) and their horticultural hybrid (Houghton's), from enriched libraries of the later two species. For B. delagoense, a tetraploid, three to 13 alleles per locus were found for native Madagascan (HO = 0.4–1.0), and one to nine in invasive Australian (HO = 0.0–1.0) samples. In addition for 91 Australian samples, only five multilocus genotypes were found (95% of individuals were of two genotypes), suggesting extensive clonality in its introduced range. These loci will be used to examine genetic diversity, hybrid origin and mating system in natural and introduced populations.

Item Type:Article
Keywords:Biological control, Bryophyllum delagoense, Bryophyllum tubiflorum, Crassulaceae, Kalanchoe, Microsatellites
Subjects:Science > Biology > Genetics > Quantitative genetics (esp. Quantitative Trait Loci (QTL) articles)
Science > Botany > Genetics
Live Archive:06 Feb 2024 23:31
Last Modified:06 Feb 2024 23:31

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