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Development of a small-plant bioassay to assess banana grown from tissue culture for consistent infection by Fusarium oxysporum f. sp. cubense

Smith, L.J. and Smith, M.K. and Tree, D. and O'Keefe, D. and Galea, V.J. (2008) Development of a small-plant bioassay to assess banana grown from tissue culture for consistent infection by Fusarium oxysporum f. sp. cubense. Australasian Plant Pathology, 37 (2). pp. 171-179.

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Article Link(s): http://dx.doi.org/10.1071/AP08006

Publisher URL: http://www.publish.csiro.au/

Abstract

Two reliable small-plant bioassays were developed using tissue-cultured banana, resulting in consistent symptom expression and infection by Fusarium oxysporum f. sp. cubense (Foc). One bioassay was based on providing a constant watertable within a closed pot and the second used free-draining pots. Culture medium for spore generation influenced infectivity of Foc. Inoculation of potted banana by drenching potting mix with a conidial suspension, consisting mostly of microconidia, few macroconidia and no chlamydospores, generated from one-quarter-strength potato dextrose agar + streptomycin sulfate, resulted in inconsistent infection. When a conidial suspension that consisted of all three spore types, microconidia, macroconidia and chlamydospores, prepared from spores generated on carnation leaf agar was used, all plants became infected, indicating that the spore type present in conidial suspensions may contribute to inconsistency of infection. Inconsistency of infection was not due to loss of virulence of the pathogen in culture. Millet grain precolonised by Foc as a source of inoculum resulted in consistent infection between replicate plants. Sorghum was not a suitable grain for preparation of inoculum as it was observed to discolour roots and has the potential to stunt root growth, possibly due to the release of phytotoxins. For the modified closed-pot system, a pasteurised potting mix consisting of equal parts of bedding sand, perlite and vermiculite plus 1 g/L Triabon slow release fertiliser was suitable for plant growth and promoted capillary movement of water through the potting mix profile. A suitable potting mix for the free-draining pot system was also developed.

Item Type:Article
Corporate Creators:Department of Employment, Economic Development and Innovation (DEEDI), Agri-Science, Crop and Food Science, Plant Science, Emerging Technologies
Business groups:Agri-Science, Crop and Food Science
Additional Information:© Australasian Plant Pathology Society. © CSIRO Publishing.
Keywords:Fusarium wilt; micropropagated banana; bacteria (microorganisms); Dianthus caryophyllus; Fusarium; Fusarium oxysporum; Fusarium oxysporum f. cubense; Solanum tuberosum.
Subjects:Plant culture > Fruit and fruit culture > Culture of individual fruits or types of fruit > Bananas
Plant pests and diseases > Individual or types of plants or trees
Plant pests and diseases > Plant pathology
Science > Microbiology
Deposited On:10 Feb 2009 05:31
Last Modified:26 Oct 2011 02:16

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