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Completion of the genome sequence of Lettuce necrotic yellows virus, type species of the genus Cytorhabdovirus

Dietzgen, R.G. and Callaghan, B. and Wetzel, T. and Dale, J.L. (2006) Completion of the genome sequence of Lettuce necrotic yellows virus, type species of the genus Cytorhabdovirus. Virus Research, 118 (1-2). pp. 16-22.

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Article Link(s): http://dx.doi.org/10.1016/j.virusres.2005.10.024

Abstract

We completed the genome sequence of Lettuce necrotic yellows virus (LNYV) by determining the nucleotide sequences of the 4a (putative phosphoprotein), 4b, M (matrix protein), G (glycoprotein) and L (polymerase) genes. The genome consists of 12,807 nucleotides and encodes six genes in the order 3′ leader-N-4a(P)-4b-M-G-L-5′ trailer. Sequences were derived from clones of a cDNA library from LNYV genomic RNA and from fragments amplified using reverse transcription-polymerase chain reaction. The 4a protein has a low isoelectric point characteristic for rhabdovirus phosphoproteins. The 4b protein has significant sequence similarities with the movement proteins of capillo- and trichoviruses and may be involved in cell-to-cell movement. The putative G protein sequence contains a predicted 25 amino acids signal peptide and endopeptidase cleavage site, three predicted glycosylation sites and a putative transmembrane domain. The deduced L protein sequence shows similarities with the L proteins of other plant rhabdoviruses and contains polymerase module motifs characteristic for RNA-dependent RNA polymerases of negative-strand RNA viruses. Phylogenetic analysis of this motif among rhabdoviruses placed LNYV in a group with other sequenced cytorhabdoviruses, most closely related to Strawberry crinkle virus.

Item Type:Article
Corporate Creators:Emerging Technologies
Additional Information:© Elsevier Science.
Keywords:Plant rhabdovirus; LNYV genomic RNA; phylogeny; virology; vesicular; stomatitis virus; polyadenylated RNAs; stunt rhabdovirus; nucleocapsid gene; net virus; identification; proteins; plant; organization; variability.
Subjects:Science > Microbiology > Virology
Science > Biology > Genetics
Plant pests and diseases
Deposited On:30 Jan 2009 03:06
Last Modified:10 Mar 2011 00:22

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