Constantinoiu, C.C. and Molloy, J.B. and Jorgensen, W.K. and Coleman, G.T. (2008) Antibody response against endogenous stages of an attenuated strain of Eimeria tenella. Veterinary Parasitology, 154 (3-4). pp. 193-204.
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Article Link(s): http://dx.doi.org/10.1016/j.vetpar.2008.03.029
Publisher URL: http://www.elsevier.com
The application of attenuated vaccines for the prevention of chicken coccidiosis has increased exponentially in recent years. In Eimeria infections, protective immunity is thought to rely on a strong cell mediated response with antibodies supposedly playing a minor role. However, under certain conditions antibodies seem to be significant in protection. Furthermore, antibodies could be useful for monitoring natural exposure of flocks to Eimeria spp. and for monitoring the infectivity of live vaccines. Our objective was to investigate the chicken antibody response to the different parasite lifecycle stages following infection with an attenuated strain of Eimeria tenella. Western blotting analysis of parasite antigens prepared from the lining of caeca infected with the attenuated strain of E. tenella revealed two dominant antigens of 32 and 34 kDa, apparently associated with trophozoites and merozoites that were present at high concentrations between 84 and 132 h post-infection. When cryosections of caeca infected with E. tenella were probed with IgY purified from immune birds the most intense reaction was observed with the asexual stages. Western blotting analysis of proteins of purified sporozoites and third generation merozoites and absorption of stage-specific antibodies from sera suggested that a large proportion of antigens is shared by the two stages. The time-courses of the antibody response to sporozoite and merozoite antigens were similar but varied depending on the inoculation regime and the degree of oocyst recirculation.
|Additional Information:||© Elsevier.|
|Keywords:||Antibody response; antigen profiles; Eimeria tenella; merozoites; sporozoites.|
|Subjects:||Veterinary medicine > Diseases of special classes of animals > Avian|
Veterinary medicine > Veterinary parasitology
Science > Microbiology > Immunology
|Deposited On:||22 Jan 2009 02:23|
|Last Modified:||26 Nov 2010 03:42|
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