Ovenden, J.R. and Street, R. and Broderick, D. (2006) New microsatellite loci for Carcharhinid sharks (Carcharhinus tilstoni and C. sorrah) and their cross-amplification in other shark species. Molecular Ecology Notes, 6 (2). pp. 415-418.
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Article Link(s): http://dx.doi.org/10.1111/j.1471-8286.2005.01254.x
Publisher URL: http://www3.interscience.wiley.com/cgi-bin/home
Twelve microsatellite DNA markers were isolated in the spot-tail shark (Carcharhinus sorrah) and nine were isolated in Australian black-tip shark (Carcharhinus tilstoni). These loci plus 18 others developed for sharks from the genera Negaprion, Ginglymostoma, Carcharodon and Isurus were tested for amplification success on four species of Carcharhinus (including C. sorrah and C. tilstoni) and four other species representing three diverse families. Cross-amplification was most common within families. Five loci were subsequently tested for polymorphism on 50 C. sorrah and 60 C. tilstoni. The number of alleles per locus was two to 24 and the average heterozygosity was 0.54 (range 0.16-0.87) for C. sorrah and 0.64 (range 0.44-0.78) for C. tilstoni. These loci may be useful tools for genetic analyses of the Carcharhinidae.
|Additional Information:||© The Authors. © Blackwell Publishing Ltd.|
|Keywords:||Black-tipped sharks; Carcharhinus sorrah; Carcharhinus tilstoni; cross-species amplification; microsatellite loci.|
|Subjects:||Aquaculture and Fisheries > Fisheries|
|Deposited On:||30 Jan 2009 05:06|
|Last Modified:||08 Sep 2010 00:50|
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