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The genetic diversity of ampeloviruses in Australian pineapples and their association with mealybug wilt disease

Gambley, C.F. and Steele, V. and Geering, A.D.W. and Thomas, J.E. (2008) The genetic diversity of ampeloviruses in Australian pineapples and their association with mealybug wilt disease. Australasian Plant Pathology, 37 (2). pp. 95-105.

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Article Link(s): http://dx.doi.org/10.1071/AP07096

Publisher URL: http://www.publish.csiro.au/

Abstract

Pineapple mealybug wilt-associated virus 1 (PMWaV-1), 2 (PMWaV-2) and -3 (PMWaV-3) have been detected in Australian commercial pineapple crops, along with a previously undescribed ampelovirus, for which the name Pineapple mealybug wilt-associated virus 5 (PMWaV-5) is proposed. Partial sequences extending from open reading frame 1b through to the heat shock protein homologue were obtained for PMWaV-1, -3 and -5. Phylogenetic analyses of selected regions of these sequences indicated that PMWaV-5 is a distinct species and most closely related to PMWaV-1. The amino acid sequence variation observed in the RNA-dependent RNA polymerase region of PMWaV-1 isolates was 95.8–98.4% and of PMWaV-3 isolates was 92.2–99.5%.

In surveys of mealybug wilt disease (MWD) affected crops, none of the four viruses was clearly associated with the disease at all survey sites. A statistically significant association (P < 0.001) between the presence of PMWaV-2 and symptoms was observed at one survey site (site 3), but the virus was at a low incidence at the remaining three survey sites. By contrast, although PMWaV-1 and -3 were equally distributed between symptomless and MWD-affected plants at site 3, there was a statistically significant (P < 0.001) association between each of these two viruses and MWD at sites 1 and 4. At site 2, there was a statistically significant (P < 0.001) association only between PMWaV-3 and MWD. PMWaV-1 was the most commonly found of the four viruses and conversely PMWaV-5 was only occasionally found. Australian isolates of PMWaV-1, -2 and -3 were transmitted by the mealybug species Dysmicoccus brevipes.

Item Type:Article
Business groups:Agri-Science, Crop and Food Science
Additional Information:© Australasian Plant Pathology Society. Reproduced with permission from © CSIRO Publishing. Access to published version may be available via Publisher’s website.
Keywords:Ananas comosus closterovirid; Closteroviridae; Closterovirus; mealybug wilt of pineapple.
Subjects:Plant culture > Fruit and fruit culture > Culture of individual fruits or types of fruit > Pineapple
Plant pests and diseases > Individual or types of plants or trees
Science > Biology > Genetics
Deposited On:28 Jan 2009 02:43
Last Modified:20 Oct 2011 06:46

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