Chinivasagam, H.H. and Corney, B.G. and Wright, L.L. and Diallo, I.S. and Blackall, P.J. (2007) Detection of Arcobacter spp. in piggery effluent and effluent-irrigated soils in southeast Queensland. Journal of Applied Microbiology, 103 (2). pp. 418-426.
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Article Link(s): http://dx.doi.org/10.1111/j.1365-2672.2007.03275.x
Publisher URL: http://www3.interscience.wiley.com/cgi-bin/home
Aims: To investigate the occurrence and levels of Arcobacter spp. in pig effluent ponds and effluent-treated soil.
Methods and Results: A Most Probable Number (MPN) method was developed to assess the levels of Arcobacter spp. in seven pig effluent ponds and six effluent-treated soils, immediately after effluent irrigation. Arcobacter spp. levels in the effluent ponds varied from 6.5 × 105 to 1.1 × 108 MPN 100 ml-1 and in freshly irrigated soils from 9.5 × 102 to 2.8 × 104 MPN g-1 in all piggery environments tested. Eighty-three Arcobacter isolates were subjected to an abbreviated phenotypic test scheme and examined using a multiplex polymerase chain reaction (PCR). The PCR identified 35% of these isolates as Arcobacter butzleri, 49% as Arcobacter cryaerophilus while 16% gave no band. All 13 nonreactive isolates were subjected to partial 16S rDNA sequencing and showed a high similarity (>99%) to Arcobacter cibarius.
Conclusions: A. butzleri, A. cryaerophilus and A. cibarius were isolated from both piggery effluent and effluent-irrigated soil, at levels suggestive of good survival in the effluent pond.
Significance and Impact of the Study: This is the first study to provide quantitative information on Arcobacter spp. levels in piggery effluent and to associate A. cibarius with pigs and piggery effluent environments.
|Corporate Creators:||Animal Science|
|Additional Information:||© Blackwell Publishing Ltd.|
|Keywords:||Arcobacter spp.; effluent; most probable number (MPN) method; pigs; soil; analysis method; detection.|
|Subjects:||Animal culture > Swine|
Science > Microbiology
Agriculture > Agriculture (General) > Soils. Soil science
|Deposited On:||04 Nov 2008 01:38|
|Last Modified:||19 Nov 2010 06:42|
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