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The introduction of transgenes to control blackheart in pineapple (Ananas Comosus L.) cv. Smooth Cayenne by microprojectile bombardment

Ko, H.L. and Campbell, P.R. and Jobin-Décor, M.P. and Eccleston, K.L. and Graham, M.W. and Smith, M.K. (2006) The introduction of transgenes to control blackheart in pineapple (Ananas Comosus L.) cv. Smooth Cayenne by microprojectile bombardment. Euphytica, 150 (3). pp. 387-395.

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Article Link(s): http://dx.doi.org/10.1007/s10681-006-9124-5

Publisher URL: http://www.springerlink.com

Abstract

A transformation technique for the introduction of transgenes to control blackheart by particle bombardment has been developed for pineapple cv. Smooth Cayenne. Leaf callus cultures capable of high frequency organogenesis with a short regeneration time were used as explant material. Gus and gfp reporter genes were used to observe and determine transient and stable expression. The ppo gene, isolated from pineapple, was introduced to control blackheart. Co-transformation occurred with constructs containing the nptII gene conferring geneticin resistance. We have recovered 15 independent transgenic gus and gfp lines each from 8 separate experiments and 22 ppo lines from 11 experiments. Gus, gfp, ppo and nptII positive plants have been regenerated, which have been shown by Southern blot analysis to be stable transgenics containing multiple copies of the introduced genes. These results show that biolistic gene delivery in pineapple can be successfully achieved at an acceptable efficiency of 0.21-1.5% for genetic improvement of 'Smooth Cayenne', the industry standard throughout the world.

Item Type:Article
Business groups:Agri-Science, Crop and Food Science, Fisheries and Aquaculture
Additional Information:© Springer Science+Business Media B.V.
Keywords:Ananas; microprojectile; particle bombardment; pineapple; polyphenol oxidase.
Subjects:Science > Science (General)
Plant culture > Fruit and fruit culture > Culture of individual fruits or types of fruit > Pineapple
Plant pests and diseases > Pest control and treatment of diseases. Plant protection > Organic plant protection. Biological control
Science > Biology > Genetics
Deposited On:03 Feb 2009 06:10
Last Modified:25 Oct 2011 04:55

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