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Host-delivered RNAi: An effective strategy to silence genes in plant parasitic nematodes

Fairbairn, D.J. and Cavallaro, A.S. and Bernard, M. and Mahalinga-Iyer, J. and Graham, M.W. and Botella, J.R. (2007) Host-delivered RNAi: An effective strategy to silence genes in plant parasitic nematodes. Planta, 226 (6). pp. 1525-1533.

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Article Link(s): http://dx.doi.org/10.1007/s00425-007-0588-x

Publisher URL: http://springerlink.com

Abstract

Root-knot nematodes (Meloidogyne spp.) are obligate, sedentary endoparasites that infect many plant species causing large economic losses worldwide. Available nematicides are being banned due to their toxicity or ozone-depleting properties and alternative control strategies are urgently required. We have produced transgenic tobacco (Nicotiana tabacum) plants expressing different dsRNA hairpin structures targeting a root-knot nematode (Meloidogyne javanica) putative transcription factor, MjTis11. We provide evidence that MjTis11 was consistently silenced in nematodes feeding on the roots of transgenic plants. The observed silencing was specific for MjTis11, with other sequence-unrelated genes being unaffected in the nematodes. Those transgenic plants able to induce silencing of MjTis11, also showed the presence of small interfering RNAs. Even though down-regulation of MjTis11 did not result in a lethal phenotype, this study demonstrates the feasibility of silencing root-knot nematode genes by expressing dsRNA in the host plant. Host-delivered RNA interference-triggered (HD-RNAi) silencing of parasite genes provides a novel disease resistance strategy with wide biotechnological applications. The potential of HD-RNAi is not restricted to parasitic nematodes but could be adapted to control other plant-feeding pests.

Item Type:Article
Additional Information:© Springer-Verlag.
Keywords:Nematode resistance; RNA interference; root knot nematodes.
Subjects:Plant pests and diseases > Pest control and treatment of diseases. Plant protection > Organic plant protection. Biological control
Science > Biology > Genetics
Deposited On:30 Oct 2008 07:01
Last Modified:19 Apr 2011 03:00

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