Devitt, L.C. and Sawbridge, T. and Holton, T.A. and Mitchelson, K. and Dietzgen, R.G. (2005) Discovery of genes associated with fruit ripening in Carica papaya using expressed sequence tags. Plant Science, 170 (2). pp. 356-363.
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Article Link(s): http://dx.doi.org/10.1016/j.plantsci.2005.09.003
Publisher URL: http://www.elsevier.com
To identify genes involved in papaya fruit ripening, a total of 1171 expressed sequence tags (ESTs) were generated from randomly selected clones of two independent fruit cDNA libraries derived from yellow and red-fleshed fruit varieties. The most abundant sequences encoded:chitinase, 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase, catalase and methionine synthase, respectively. DNA sequence comparisons identified ESTs with significant similarity to genes associated with fruit softening, aroma and colour biosynthesis. Putative cell wall hydrolases, cell membrane hydrolases, and ethylene synthesis and regulation sequences were identified with predicted roles in fruit softening. Expressed papaya genes associated with fruit aroma included isoprenoid biosynthesis and shikimic acid pathway genes and proteins associated with acyl lipid catabolism. Putative fruit colour genes were identified due to their similarity with carotenoid and chlorophyll biosynthesis genes from other plant species.
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|Keywords:||Expressed sequence tag; Carica papaya; library; fruit ripening; carotenoid.|
|Subjects:||Science > Biology > Genetics|
Plant culture > Fruit and fruit culture > Culture of individual fruits or types of fruit
|Deposited On:||24 Dec 2007|
|Last Modified:||16 Mar 2011 00:54|
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