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Comparison of the indirect haemagglutination and gel diffusion test for serotyping Haemophilus parasuis

Turni, C. and Blackall, P.J. (2005) Comparison of the indirect haemagglutination and gel diffusion test for serotyping Haemophilus parasuis. Veterinary Microbiology, 106 (1-2). pp. 145-151.

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Article Link(s): http://dx.doi.org/10.1016/j.vetmic.2004.12.019

Publisher URL: http://www.elsevier.com

Abstract

The aim of this study was to compare the use of indirect haemagglutination (IHA) and gel diffusion (GD) tests for serotyping Haemophilus parasuis by the Kielstein-Rapp-Gabrielson scheme. All 15 serovar reference strains, 72 Australian field isolates, nine Chinese field isolates, and seven isolates from seven experimentally infected pigs were evaluated with both tests. With the IHA test, 14 of the 15 reference strains were correctly serotyped – with serovar 10 failing to give a titre with serovar 10 antiserum.

In the GD test, 13 reference strains were correctly serotyped – with antigen from serovars 7 and 8 failing to react with any antiserum. The IHA methodology serotyped a total of 45 of 81 field isolates while the GD methodology serotyped a total of 48 isolates. For 29 isolates, the GD and IHA methods gave discordant results. It was concluded that the IHA is a good additional test for the serotyping of H. parasuis by the KRG scheme if the GD methodology fails to provide a result or shows unusual cross-reactions.

Item Type:Article
Corporate Creators:Animal Science
Additional Information:Author version © Queensland Department of Primary Industries and Fisheries.. Reproduced in accordance with the copyright policy of the publisher. © Elsevier. Access to published version may be available via Publisher’s website.
Keywords:Hemophilus parasuis; indirect haemagglutination; IHA; gel diffusion; GD; serotyping; pigs.
Subjects:Veterinary medicine > Diseases of special classes of animals > Swine
Veterinary medicine > Veterinary bacteriology
Deposited On:15 Aug 2006
Last Modified:10 Sep 2010 03:21

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