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A simplified PCR test for early detection of dwarf off-types in micropropagated Cavendish bananas (Musa spp. AAA)

Ramagea, C.M. and Borda, A.M. and Hamill, A.M. and Smith, M.K. (2004) A simplified PCR test for early detection of dwarf off-types in micropropagated Cavendish bananas (Musa spp. AAA). Scientia Horticulturae, 103 (1). pp. 145-151.

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Article Link(s): http://dx.doi.org/10.1016/j.scienta.2004.04.015

Publisher URL: http:// www.elsevier.com

Abstract

The dwarf somaclonal variant is a major problem affecting micropropagation of the banana cultivar Williams (Musa spp. AAA; subgroup Cavendish). This problem arises from genetic changes that occur during the tissue culture process. Early identification of this problem is difficult and propagators must wait until plants are ex vitro in order to visualise the dwarfism phenotype. In this study, we have improved a SCAR-based molecular diagnostic technique, developed by Damasco et al. [Acta Hortic. 461 (1997) 157], for the early identification of dwarf off-types. We have included a positive internal control in a multiplex PCR and adapted the technique for use with small amounts of fresh in vitro leaf material as PCR template. The control product is a 500 bp fragment from 18S rRNA and is amplified in all tissues irrespective of phenotype. The use of small in vitro leaf material removing the need for genomic DNA extraction.

Item Type:Article
Additional Information:© Elsevier.
Keywords:Banana; dwarf off-type; diagnostic; SCAR marker; Multiplex PCR; in vitro.
Subjects:Plant culture > Fruit and fruit culture > Culture of individual fruits or types of fruit > Bananas
Plant culture > Propagation
Deposited On:24 Dec 2007
Last Modified:14 Mar 2011 03:19

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