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The Introduction of Transgenes to Control Blackheart in Pineapple: Biolistics Vs Agrobacterium Transformation, in 'Contributing to a Sustainable Future'

Ko, L. and Hardy, V. and Jobin-Decor, M. and Campbell, P. and Eccleston, K. and Graham, M. and Smith, M. (2005) The Introduction of Transgenes to Control Blackheart in Pineapple: Biolistics Vs Agrobacterium Transformation, in 'Contributing to a Sustainable Future'. In: "International Association for Plant Tissue Culture and Biotechnology" (IAPTC) Conference (Australian Branch- 7th National Meeting), 21-24 September, Perth (WA);.

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Publisher URL: http://www.iapb-stl.org/

Abstract

Techniques for the introduction of transgenes to control blackheart by particle bombardment and Agrobacterium co-transformation have been developed for pineapple cv. Smooth Cayenne. Polyphenol oxidase (PPO) is the enzyme responsible for blackheart development in pineapple fruit following chilling injury. Sense, anti-sense and hairpin constructs were used as a means to suppress PPO expression in plants. Average transformation efficiency for biolistics was approximately 1% and for Agrobacterium was approximately 1.5%. These results were considered acceptable given the high regeneration potential of between 80-90% from callus cultures. Southern blot analysis revealed stable integration of transgenes with lower copy number found in plants transformed with Agrobacterium compared to those transformed by biolistics. Over 5000 plants from 55 transgenic lines are now undergoing field evaluation in Australia

Item Type:Conference or Workshop Item (Paper)
Additional Information:© The State of Queensland, Department of Primary Industries & Fisheries. Copyright protects this publication. Except for purposes permitted by the Copyright Act 1968, reproduction by whatever means is prohibited without prior written permission of the Department of Primary Industries & Fisheries, Queensland.
Keywords:Ananas comosus; genetic transformation; gene silencing; postharvest disorder.
Subjects:Plant culture > Fruit and fruit culture > Culture of individual fruits or types of fruit > Pineapple
Plant pests and diseases > Individual or types of plants or trees
Plant pests and diseases > Pest control and treatment of diseases. Plant protection > Organic plant protection. Biological control
Science > Biology > Genetics
Deposited On:20 Aug 2007
Last Modified:20 Apr 2011 01:48

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