Higgins, Colleen M. and Hall, Rhonda M. and Mitter, Neena and Cruickshank, Alan and Dietzgen, Ralf G. (2004) Peanut stripe potyvirus resistance in peanut (Arachis hypogaea L.) plants carrying viral coat protein gene sequences. Transgenic Research, 13 (1). pp. 59-67.
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Article Link(s): http://dx.doi.org/10.1023/B:TRAG.0000017166.29458....
Publisher URL: http://www.springerlink.com
Peanut (Arachis hypogaea L.) lines exhibiting high levels of resistance to peanut stripe virus (PStV) were obtained following microprojectile bombardment of embryogenic callus derived from mature seeds. Fertile plants of the commercial cultivars Gajah and NC7 were regenerated following co-bombardmentwith the hygromycin resistance gene and one of two forms of the PStV coat protein (CP) gene, an untranslatable, full length sequence (CP2) or a translatable gene encoding a CP with an N-terminal truncation (CP4). High level resistance to PStV was observed for both transgenes when plants were challenged with the homologous virus isolate. The mechanism of resistance appears to be RNA-mediated, since plants carrying either the untranslatable CP2 or CP4 had no detectable protein expression, but were resistant or immune (no virus replication). Furthermore, highly resistant, but not susceptible CP2 T0 plants contained transgene-specific small RNAs. These plants now provide important germplasm for peanut breeding, particularly in countries where PStV is endemic and poses a major constraint to peanut production.
|Corporate Creators:||Department of Employment, Economic Development and Innovation (DEEDI), Agri-Science, Crop and Food Science|
|Business groups:||Agri-Science, Crop and Food Science|
|Additional Information:||© Springer.|
|Keywords:||Peanut; potyvirus; RNA-mediated virus resistance; transformation.|
|Subjects:||Plant culture > Fruit and fruit culture > Nuts|
Plant pests and diseases > Pest control and treatment of diseases. Plant protection > Organic plant protection. Biological control
Plant pests and diseases > Individual or types of plants or trees
Science > Biology > Genetics
|Deposited On:||20 Aug 2007|
|Last Modified:||25 Oct 2011 03:45|
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