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Nucleocapsid gene variability reveals two subgroups of Lettuce necrotic yellows virus

Callaghan, B. and Dietzgen, R. G. (2005) Nucleocapsid gene variability reveals two subgroups of Lettuce necrotic yellows virus. Archives of Virology, 150 (8). pp. 1661-1667.

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Article Link(s): http://dx.doi.org/10.1007/s00705-005-0528-7

Publisher URL: http://www.springerlink.com

Abstract

The complete nucleocapsid (N) genes of eight Australian isolates of Lettuce necrotic yellows virus (LNYV) were amplified by reverse transcription PCR, cloned and sequenced. Phylogenetic analyses of these sequences revealed two distinct subgroups of LNYV isolates. Nucleotide sequences within each subgroup were more than 96% identical but heterogeneity between groups was about 20% at the nucleotide sequence level. However, less than 4% heterogeneity was noted at the amino acid level, indicating mostly third nucleotide position changes and a strong conservation for N protein function. There was no obvious geographical or temporal separation of the subgroups in Australia.

Item Type:Article
Additional Information:© Springer Verlag.
Keywords:Nucleocapsid; genes; isolates; lettuce necrotic yellow virus; (LNYV); reverse transcription; PCR; phylogenetic; nucleotide sequences.
Subjects:Science > Microbiology > Virology
Plant pests and diseases > Plant pathology
Science > Biology > Genetics
Deposited On:24 Dec 2007
Last Modified:08 Jun 2011 04:34

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