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A precision apparatus, with solid phase micro-extraction monitoring capability, for incorporation studies of gaseous precursors into insect-derived metabolites

Fletcher, M.T. and Wood, B.J. and Schwartz, B.D. and Rahm, F. and Lambert, L.K. and Brereton, I.M. and Moore, C.J. and De Vossa, J.J. and Kitching, W. (2004) A precision apparatus, with solid phase micro-extraction monitoring capability, for incorporation studies of gaseous precursors into insect-derived metabolites. ARKIVOC (Part (x): Commemorative Issue in Honor of Prof. Rodney W. Rickards on the occasion of his 70th anniversary). pp. 109-117.

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Publisher URL: http://www.arkat-usa.org

Abstract

An apparatus is described that facilitates the determination of incorporation levels of isotope labelled, gaseous precursors into volatile insect-derived metabolites. Atmospheres of varying gas compositions can be generated by evacuation of a working chamber followed by admission of the required levels of component gases, using a precision, digitised pressure read-out system.

Insects such as fruit-flies are located initially in a small introduction chamber, from which migration can occur downwards into the working chamber. The level of incorporation of labelled precursors is continuously assayed by the Solid Phase Micro Extraction (SPME) technique and GC-MS analyses. Experiments with both Bactrocera species (fruit-flies) and a parasitoid wasp, Megarhyssa nortoni nortoni (Cresson) and oxygen-18 labelled dioxygen illustrate the utility of this system. The isotope effects of oxygen-18 on the carbon-13 NMR spectra of 1,7- dioxaspiro[5,5]undecane are also described.

Item Type:Article
Additional Information:ARKIVOC. Access to the electronic edition of ARKIVOC (www.arkat-usa.org) is free to all anywhere in the world. Downloading is permitted freely of single papers from the HTML or PDF version and of complete issues or volumes from the PDF version.
Keywords:Insect chamber; incorporation studies; metabolites; spiroacetals; oxygen-18 labelling; isotope effects; carbon-13 NMR spectra; carbon monoxide.
Subjects:Technology > Technology (General)
Science > Science (General)
Science > Zoology > Invertebrates > Insects
Deposited On:24 Jul 2007
Last Modified:01 Apr 2011 05:00

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