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Abacá mosaic virus: a distinct strain of Sugarcane mosaic virus

Gambley, C.F. and Thomas, J.E. and Magnaye, L.V. and Herradura, L. (2004) Abacá mosaic virus: a distinct strain of Sugarcane mosaic virus. Australasian Plant Pathology, 33 (4). pp. 475-484.

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Article Link(s): http://dx.doi.org/10.1071/AP04050

Publisher URL: http://www.publish.csiro.au/

Abstract

Abacá mosaic virus (AbaMV) is related to members of the sugarcane mosaic virus subgroup of the genus Potyvirus. The ~2 kb 3′ terminal region of the viral genome was sequenced and, in all areas analysed, found to be most similar to Sugarcane mosaic virus (SCMV) and distinct from Johnsongrass mosaic virus (JGMV), Maize dwarf mosaic virus (MDMV) and Sorghum mosaic virus (SrMV). Cladograms of the 3′ terminal region of the NIb protein, the coat protein core and the 3′ untranslated region showed that AbaMV clustered with SCMV, which was a distinct clade and separate from JGMV, MDMV and SrMV. The N-terminal region of the AbaMV coat protein had a unique amino acid repeat motif different from those previously published for other strains of SCMV. The first experimental transmission of AbaMV from abacá (Musa textilis) to banana (Musa sp.), using the aphid vectors Rhopalosiphum maidis and Aphis gossypii, is reported. Polyclonal antisera for the detection of AbaMV in western blot assays and ELISA were prepared from recombinant coat protein expressed in E. coli. A reverse transcriptase PCR diagnostic assay, with microtitre plate colourimetric detection, was developed to discriminate between AbaMV and Banana bract mosaic virus, another Musa-infecting potyvirus. Sequence data, host reactions and serological relationships indicate that AbaMV should be considered a distinct strain of SCMV, and the strain designation SCMV-Ab is suggested.

Item Type:Article
Corporate Creators:Department of Employment, Economic Development and Innovation (DEEDI), Agri-Science, Crop and Food Science, Horticulture and Forestry Science
Business groups:Agri-Science, Crop and Food Science
Additional Information:Reproduced with permission from © CSIRO Publishing. Access to published version may be available via Publisher’s website.
Keywords:Abacá; banana; RT-PCR diagnosis; ELISA; aphid transmission; Potyvirus.
Subjects:Plant pests and diseases > Plant pathology
Deposited On:19 Jul 2007
Last Modified:08 Jun 2015 15:50

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