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A lack of predatory interaction between rumen ciliate protozoa and Shiga-toxin producing Escherichia coli

Burow, L.C. and Gobius, K.S. and Vanselow, B.A. and Klieve, A.V. (2005) A lack of predatory interaction between rumen ciliate protozoa and Shiga-toxin producing Escherichia coli. Letters in Applied Microbiology, 40 . pp. 117-122.

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Article Link(s): http://dx.doi.org/10.1111/j.1472-765X.2004.01642.x

Publisher URL: http://www.blackwell-synergy.com

Abstract

Aims: To investigate interactions between rumen protozoa and Shiga toxin-producing Escherichia coli (STEC) and to ascertain whether it is likely that rumen protozoa act as ruminant hosts for STEC.

Methods and Results: The presence of stx genes in different microbial fractions recovered from cattle and sheep rumen contents and faeces was examined using PCR. In animals shedding faecal STEC, stx genes were not detected in the rumen bacterial or rumen protozoal fractions. Direct interactions between ruminal protozoa and STEC were investigated by in vitro co-incubation. Rumen protozoa did not appear to ingest STEC, a STEC lysogen or non-STEC E. coli populations when co-incubated.

Conclusions: The ruminal environment is unlikely to be a preferred habitat for STEC. Bacterial grazing by rumen protozoa appears to have little, if any, effect on STEC populations.

Significance and Impact of the Study: This study indicates that ruminal protozoa are unlikely to be a major factor in the survival of STEC in ruminants. They appear as neither a host that protects STEC from the ruminal environment nor a predator that might reduce STEC numbers.

Item Type:Article
Additional Information:© Blackwell Publishing Ltd. Information for the published version of the paper, as published in the print edition of Letters in Applied Microbiology, is available on the Blackwell Synergy online delivery service, accessible via the journal's website at http://www.blackwell-synergy.com.
Keywords:Cattle; E. coli; PCR; rumen; sheep.
Subjects:Science > Microbiology > Microorganisms in the animal body
Veterinary medicine > Veterinary microbiology
Deposited On:10 Mar 2005
Last Modified:07 Jun 2011 05:37

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