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Characterization, development and multiplexing of microsatellite markers in three commercially exploited reef fish and their application for stock identification

Taillebois, Laura and Dudgeon, Christine and Maher, Safia and Crook, David A. and Saunders, Thor M. and Barton, Diane P. and Taylor, Jonathan A. and Welch, David J. and Newman, Stephen J. and Travers, Michael J. and Saunders, Richard J. and Ovenden, Jennifer (2016) Characterization, development and multiplexing of microsatellite markers in three commercially exploited reef fish and their application for stock identification. PeerJ, 4 . e2418. ISSN 2167-8359

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Article Link(s): https://doi.org/10.7717/peerj.2418

Abstract

Thirty-four microsatellite loci were isolated from three reef fish species; golden snapper Lutjanus johnii, blackspotted croaker Protonibea diacanthus and grass emperor Lethrinus laticaudis using a next generation sequencing approach. Both IonTorrent single reads and Illumina MiSeq paired-end reads were used, with the latter demonstrating a higher quality of reads than the IonTorrent. From the 1–1.5 million raw reads per species, we successfully obtained 10–13 polymorphic loci for each species, which satisfied stringent design criteria. We developed multiplex panels for the amplification of the golden snapper and the blackspotted croaker loci, as well as post-amplification pooling panels for the grass emperor loci. The microsatellites characterized in this work were tested across three locations of northern Australia. The microsatellites we developed can detect population differentiation across northern Australia and may be used for genetic structure studies and stock identification.

Item Type:Article
Business groups:Animal Science
Keywords:Next-generation sequencing Lutjanus johnii Protonibea diacanthus Multiplex panels Microsatellite markers Lethrinus laticaudis
Subjects:Science > Biology > Genetics
Aquaculture and Fisheries > Fisheries > Fishery research
Deposited On:07 Sep 2016 07:01
Last Modified:07 Sep 2016 07:01

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