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Cryopreservation of avocado (Persea Americana Mill.) using somatic embryos

O’Brien, C. and Parisi, A. and Lim Yiing Yuan, J. and Constantin, M. and Mitter, N. (2016) Cryopreservation of avocado (Persea Americana Mill.) using somatic embryos. Acta Horticulturae (1113). pp. 265-270. ISSN 0567-7572

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Article Link(s): http://dx.doi.org/10.17660/ActaHortic.2016.1113.39

Abstract

Avocado genetic resources are currently maintained in the form of field repositories at great cost and risk of natural disasters, pest and diseases. Cryopreservation offers a necessary, complimentary method that is safe, cost-effective and long-term. However, long-term maintenance and regeneration of plantlets from avocado somatic embryos has been a major barrier in the development of new avocado cultivars. In this study, two protocols for vitrification-based cryopreservation of avocado somatic embryos were investigated. Globular somatic embryos of two avocado cultivars were tested, revealing cultivar-dependent differences in desiccation tolerance and subsequent freezing resistance, possibly attributed to their size and culture age. A two-step regeneration system, involving an intermediate liquid phase step between subcultures in solid medium, significantly enhanced shoot development from somatic embryo tissue. This work will add considerable value towards cryopreservation of avocado somatic embryos for germplasm conservation and the generation of new and improved avocado cultivars.

Item Type:Article
Business groups:Horticulture and Forestry Science
Keywords:Droplet-vitrification; Loading solution; PVS2; Vitrification
Subjects:Science > Botany > Genetics
Plant culture > Harvesting, curing, storage
Plant culture > Fruit and fruit culture
Deposited On:05 Jul 2016 03:28
Last Modified:05 Jul 2016 03:28

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