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Molecular analysis of RNA1 and RNA2 sequences from a betanodavirus isolated from giant grouper (Epinephelus lanceolatus) in Australia

Agnihotri, Kalpana and Pease, Brad and Chong, Roger (2016) Molecular analysis of RNA1 and RNA2 sequences from a betanodavirus isolated from giant grouper (Epinephelus lanceolatus) in Australia. Virology Reports, 6 . pp. 25-31. ISSN 22146695

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Article Link(s): http://dx.doi.org/10.1016/j.virep.2016.05.001

Abstract

Betanodavirus infections have a significant impact through direct losses and trade restrictions for aquaculture sectors in Australia. The giant grouper, Epinephelus lanceolatus, is a high-value, fast-growing species with significant aquaculture potential. With subacute to chronic mortalities reported from a commercial aquaculture facility in northern Queensland, the viral nervous necrosis in the affected fish was confirmed using a RT-qPCR followed by virus isolation using the SSN-1 cell line. The RNA1 and RNA2 segments were sequenced and nucleotide sequences were compared with betanodavirus sequences from GenBank. Phylogenetic analysis revealed that both these sequences clustered with sequences representing red spotted grouper nervous necrosis virus genotype and showed high sequence identity to virus sequences affecting other grouper species. This is the first report confirming infection by betanodavirus in E. lanceolatus from Australia with successful isolation of the virus in a cell culture system, and analysis of nearly full length RNA1 and RNA2 sequences.

Item Type:Article
Business groups:Biosecurity Queensland
Keywords:Betanodavirus Giant grouper Australia
Subjects:Science > Biology > Genetics
Veterinary medicine > Veterinary virology
Aquaculture and Fisheries > Aquaculture > Fish culture
Aquaculture and Fisheries > Aquaculture > Fish culture > Diseases and adverse factors
Deposited On:01 Jun 2016 00:41
Last Modified:30 Aug 2016 02:57

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