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Inheritance and Characterization of Strong Resistance to Phosphine in Sitophilus oryzae(L.)

Nguyen, Tam T. and Collins, Patrick J. and Ebert, Paul R. (2015) Inheritance and Characterization of Strong Resistance to Phosphine in Sitophilus oryzae(L.). PLoS ONE, 10 (4). e0124335.

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Article Link(s): http://dx.doi.org/10.1371%2Fjournal.pone.0124335

Abstract

Sitophilus oryzae (Linnaeus) is a major pest of stored grain across Southeast Asia and is of increasing concern in other regions due to the advent of strong resistance to phosphine, the fumigant used to protect stored grain from pest insects. We investigated the inheritance of genes controlling resistance to phosphine in a strongly resistant S. oryzae strain (NNSO7525) collected in Australia and find that the trait is autosomally inherited and incompletely recessive with a degree of dominance of -0.66. The strongly resistant strain has an LC50 52 times greater than a susceptible reference strain (LS2) and 9 times greater than a weakly resistant strain (QSO335). Analysis of F2 and backcross progeny indicates that two or more genes are responsible for strong resistance, and that one of these genes, designated Sorph1, not only contributes to strong resistance, but is also responsible for the weak resistance phenotype of strain QSO335. These results demonstrate that the genetic mechanism of phosphine resistance in Soryzae is similar to that of other stored product insect pests. A unique observation is that a subset of the progeny of an F1 backcross generation are more strongly resistant to phosphine than the parental strongly resistant strain, which may be caused by multiple alleles of one of the resistance genes.

Item Type:Article
Business groups:Crop and Food Science
Subjects:Science > Botany > Genetics
Plant culture > Field crops > Grain. Cereals
Plant pests and diseases
Deposited On:13 Jul 2015 02:16
Last Modified:13 Jul 2015 02:16

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