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Human-associated fluoroquinolone-resistant Escherichia coli clonal lineages, including ST354, isolated from canine feces and extraintestinal infections in Australia

Guo, SiYu and Wakeham, David and Brouwers, Huub J. M. and Cobbold, Rowland N. and Abraham, Sam and Mollinger, Joanne L. and Johnson, James R. and Chapman, Toni A. and Gordon, David M. and Barrs, Vanessa R. and Trott, Darren J. (2015) Human-associated fluoroquinolone-resistant Escherichia coli clonal lineages, including ST354, isolated from canine feces and extraintestinal infections in Australia. Microbes and Infection, 17 (4). pp. 266-274.

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Article Link(s): http://dx.doi.org/10.1016/j.micinf.2014.12.016

Publisher URL: http://www.sciencedirect.com/science/article/pii/S1286457914003384

Abstract

Phylogenetic group D extraintestinal pathogenic Escherichia coli (ExPEC), including O15:K52:H1 and clonal group A, have spread globally and become fluoroquinolone-resistant. Here we investigated the role of canine feces as a reservoir of these (and other) human-associated ExPEC and their potential as canine pathogens. We characterized and compared fluoroquinolone-resistant E. coli isolates originally identified as phylogenetic group D from either the feces of hospitalized dogs (n = 67; 14 dogs) or extraintestinal infections (n = 53; 33 dogs). Isolates underwent phylogenetic grouping, random amplified polymorphic DNA (RAPD) analysis, virulence genotyping, resistance genotyping, human-associated ExPEC O-typing, and multi-locus sequence typing. Five of seven human-associated sequence types (STs) exhibited ExPEC-associated O-types, and appeared in separate RAPD clusters. The largest subgroup (16 fecal, 26 clinical isolates) were ST354 (phylogroup F) isolates. ST420 (phylogroup B2); O1-ST38, O15:K52:H1-ST393, and O15:K1-ST130 (phylogroup D); and O7-ST457, and O1-ST648 (phylogroup F) were also identified. Three ST-specific RAPD sub-clusters (ST354, ST393, and ST457) contained closely related isolates from both fecal or clinical sources. Genes encoding CTX-M and AmpC β-lactamases were identified in isolates from five STs. Major human-associated fluoroquinolone-resistant ± extended-spectrum cephalosporin-resistant ExPEC of public health importance may be carried in dog feces and cause extraintestinal infections in some dogs.

Item Type:Article
Business groups:Biosecurity Queensland
Keywords:Extraintestinal pathogenic Escherichia coli Fluoroquinolone-resistant Extended-spectrum cephalosporin-resistant Phylogenetic group D
Subjects:Science > Microbiology
Science > Microbiology > Microorganisms in the animal body
Veterinary medicine > Veterinary microbiology
Deposited On:13 Jul 2015 03:36
Last Modified:13 Jul 2015 03:36

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