Bejerman, Nicolás and Giolitti, Fabián and de Breuil, Soledad and Trucco, Verónica and Nome, Claudia and Lenardon, Sergio and Dietzgen, Ralf G. (2015) Complete genome sequence and integrated protein localization and interaction map for alfalfa dwarf virus, which combines properties of both cytoplasmic and nuclear plant rhabdoviruses. Virology, 483 . pp. 275-283. ISSN 0042-6822
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Article Link(s): http://dx.doi.org/10.1016/j.virol.2015.05.001
Summary We have determined the full-length 14,491-nucleotide genome sequence of a new plant rhabdovirus, alfalfa dwarf virus (ADV). Seven open reading frames (ORFs) were identified in the antigenomic orientation of the negative-sense, single-stranded viral RNA, in the order 3′-N-P-P3-M-G-P6-L-5′. The ORFs are separated by conserved intergenic regions and the genome coding region is flanked by complementary 3′ leader and 5′ trailer sequences. Phylogenetic analysis of the nucleoprotein amino acid sequence indicated that this alfalfa-infecting rhabdovirus is related to viruses in the genus Cytorhabdovirus. When transiently expressed as GFP fusions in Nicotiana benthamiana leaves, most ADV proteins accumulated in the cell periphery, but unexpectedly P protein was localized exclusively in the nucleus. ADV P protein was shown to have a homotypic, and heterotypic nuclear interactions with N, P3 and M proteins by bimolecular fluorescence complementation. ADV appears unique in that it combines properties of both cytoplasmic and nuclear plant rhabdoviruses.
|Business groups:||Horticulture and Forestry Science|
|Keywords:||Genome structure Cytorhabdovirus Nucleorhabdovirus Protein localization Protein–protein interactions Phosphoprotein|
|Subjects:||Science > Biology > Genetics|
|Deposited On:||06 Jul 2015 01:37|
|Last Modified:||06 Jul 2015 01:37|
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